Regulation of endogenous gene expression in cells using zinc finger proteins

A method for modulating expression of an endogenous gene in a cell, wherein the method comprises:

• a) administering to the cell a nucleic acid molecule comprising a polynucleotide sequence encoding an engineered zinc finger protein, wherein the zinc finger protein comprises a plurality of zinc fingers and further wherein the zinc finger protein is partially randomized;
• and
• b) maintaining the cell under conditions in which the engineered zinc finger protein is expressed and binds to a target site in the gene.