Stirred-tank reactor system
AssigneesBaxter International Inc., Baxter Healthcare S.A., HyClone Laboratories, Inc.
InventorsKurt Kunas, Robert Oakley, Fauad Hasan, Michael Goodwin, Jeremy Larsen, Nephi Jones
Life of this patent
Estimating the term…
Abstract
The present invention relates to a stirred-tank reactor system and methods of preparing such systems. The present invention further encompasses the use of the stirred-tank reactor system as a disposable bioreactor and in kits with disposable elements.
Claims26
A reactor system, the system comprising:
- a container;
- a rotational assembly in sealed cooperation with an opening of the container, the rotational assembly comprising a rotatable hub adapted to receive and releasably couple with a drive shaft, such that when the drive shaft is operatively coupled with the rotatable hub, rotation of the drive shaft facilitates a corresponding rotation of the rotatable hub.
A reactor system, the system comprising:
- a container;
- and
- a sparger assembly disposed within the container, the sparger assembly comprising a flexible sheet of permeable material and a sparger conduit.
A bioreactor system, the system comprising:
- a frame support coupled with a drive motor;
- a flexible bag disposed within a housing of the frame support, the flexible bag comprising one or more ports for introducing a cell culture and a medium into the flexible bag;
- a rotational assembly coupled with a bracket of the frame support and in sealed cooperation with an opening of the flexible bag, the rotational assembly comprising a hub adapted to house and couple with a drive shaft of the drive motor;
- and
- an impeller coupled with the hub for agitating the cell culture and medium, the impeller disposed within the flexible bag and adapted to couple with the drive shaft.
A method for manufacturing a reactor system, the method comprising:
- coupling a container with a rotational assembly, the rotational assembly in sealed cooperation with an opening of the container, the rotational assembly comprising a hub adapted to house and couple with a drive shaft;
- coupling an impeller with the hub, the impeller disposed within the container;
- and
- sterilizing the reactor system.
A method for preparing a reactor system, the method comprising:
- coupling a casing of a rotational assembly of the reactor system to a frame bracket;
- placing a container of the reactor system at least partially within a frame housing;
- inserting a drive shaft into a hub of the rotational assembly, the hub disposed within the casing of the rotational assembly between a bearing and the casing;
- coupling a distal end of the drive shaft to an impeller, the impeller disposed within the container and coupled with the hub;
- and
- introducing a reaction component into the container via a port.
A reactor system kit, the kit comprising:
- a reactor system, the reactor system comprising:
- a container;
- a rotational assembly in sealed cooperation with an opening of the container, the rotational assembly comprising a hub adapted to house and couple with a drive shaft;
- and
- an impeller coupled with the hub, the impeller disposed within the container and adapted to couple with the drive shaft;
- and
- instructions for use.
Dependencies are inferred from the claim text.