Device and method of use for detection and characterization of microorganisms and microparticles
AssigneeBioForce Nanosciences, Inc.
InventorsEric Henderson, Saju Nettikadan, Curtis Mosher, James Johnson
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Abstract
The present invention includes a method and apparatus for the detection of a microorganism or microparticle.
Claims57
An apparatus for capturing a microorganism or microparticle comprising:
- a solid support comprising a surface adapted for use in a scanning probe microscope;
- a material deposited in a plurality of discrete domains on the surface, wherein the material is capable of interacting with the microorganism or the microparticle, and wherein exposure of the apparatus to a sample which can contain the microorganism or the microparticle causes the microorganism or microparticle to interact with the material.
A method for detecting a microorganism or microparticle in a sample comprising:
- providing a substrate with a surface;
- depositing a material on the surface in a plurality of discrete domains, wherein the material is capable of interacting with the microorganism or microparticle;
- exposing the material to the sample;
- and
- detecting an interaction between the material and the microorganism by imaging the interaction.
A method for amplifying a nucleic acid within a microorganism comprising:
- (a) providing a substrate with a surface;
- (b) depositing a material on the surface, wherein the material has a capacity to interact with the microorganism;
- (c) exposing the material to a sample which can contain the microorganism, wherein the microorganism comprises a nucleic acid, and wherein the microorganism interacts with the material;
- and
- (d) amplifying the nucleic acid of step c in a polymerase chain reaction.
A method for screening antibodies capable of capturing particulate antigens comprising:
- providing a substrate with a surface;
- depositing an antibody onto the surface;
- exposing the antibody to a component comprising a particulate antigen;
- and
- measuring the interaction between the antibody and the particulate antigen using scanning force microscopy.
A method of screening for an antiviral agent comprising:
- providing a substrate with a surface;
- depositing a capture reagent onto the surface;
- exposing the surface to sample which can contain a virus and an antiviral agent;
- and
- detecting the virus that interacts with the capture reagent using scanning probe microscopy.
A method of screening for an antiviral agent comprising:
- (a) providing a probe suitable for use in atomic force microscopy;
- (b) attaching a virus to the probe;
- (c) contacting the probe with a surface, wherein the surface comprises a material that can interact with the virus;
- (d) contacting the receptor and the virus of step (c) with a sample that can contain an antiviral agent;
- and
- (e) detecting a change in the interaction between the virus and the receptor, wherein the change is correlated with the presence of an antiviral agent.
A method for detecting the presence of an antibody from an animal comprising:
- providing a substrate with a surface;
- depositing the antibody on the surface, wherein the antibody is derived from the animal, and wherein the antibody is capable of interacting with a microorganism;
- exposing the antibody to the microorganism;
- and
- detecting an interaction between the antibody and the microorganism.
A method for detecting the presence of a microorganism from an animal comprising:
- providing a substrate with a surface;
- depositing an antibody on the surface, wherein the antibody is derived from the animal, and wherein the antibody is capable of interacting with the microorganism;
- exposing the antibody to the microorganism;
- and
- detecting an interaction between the antibody and the microorganism.
An apparatus for detecting a microorganism or microparticle comprising:
- a solid support comprising a surface adapted for use in diffraction assay;
- a material deposited in a plurality of linear arrays on the surface, wherein the material is capable of interacting with the microorganism or the microparticle, and wherein exposure of the apparatus to a sample which can contain the microorganism or the microparticle causes the microorganism or microparticle to interact with the material.
Dependencies are inferred from the claim text.