Hybrid automated continuous nucleic acid and protein analyzer using real-time PCR and liquid bead arrays
AssigneeThe Regents of the University of California
InventorsShanavaz Nasarabadi, Richard Langlois, Billy Colston, Evan Skowronski, Fred Milanovich
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Abstract
A nucleic acid assay system for analyzing a sample using a reagent. A sample and reagent delivery unit is operatively connected to a thermal cycler for delivering the sample and the reagent to the thermal cycler. A hybridization chamber is operatively connected to the thermal cycler. A flow cytometer is operatively connected to the hybridization chamber.
Claims54
A nucleic acid assay apparatus for analyzing a sample using a reagent, comprising:
- a thermal cycler,
- a sample and reagent delivery unit operatively connected to said thermal cycler for delivering the sample and the reagent to said thermal cycler,
- a hybridization chamber operatively connected to said thermal cycler, and
- a flow cytometer operatively connected to said hybridization chamber.
A nucleic acid assay apparatus for analyzing a sample using a reagent, comprising:
- thermal cycler means,
- sample and reagent delivery means operatively connected to said thermal cycler means for delivering the sample and the reagent to said thermal cycler means,
- hybridization means operatively connected to said thermal cycler means, and
- flow cytometer means operatively connected to said hybridization means.
A nucleic acid assay method for analyzing a sample using a reagent, comprising the steps of:
- providing a thermal cycler,
- providing a hybridization unit,
- providing a flow cytometer,
- transporting the sample and the reagent to said thermal cycler for amplification, and
- analyzing the sample with said flow cytometer utilize beads with each bead having a unique spectral address.
Dependencies are inferred from the claim text.