Methods to culture circovirus
AssigneeUniversity of Saskatchewan
InventorsQiang Liu, Suresh Tikoo, Philip Willson, Lorne Babiuk
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Abstract
The present invention relates to methods for culturing circovirus and in particular, porcine circovirus. The present invention provides compositions and methods for culturing porcine circovirus in mammalian cells expressing mammalian adenovirus E1 function.
Claims34
A method for culturing a mammalian circovirus comprising:
- a) obtaining mammalian cells expressing a mammalian adenovirus E1 function, wherein said cells are permissive for mammalian circovirus replication;
- b) introducing the mammalian circovirus genome, or a portion thereof capable of replication, into said mammalian cells;
- and
- c) culturing said mammalian cells under conditions suitable for replication of said mammalian circovirus.
A recombinant mammalian cell that expresses a mammalian adenovirus E1 function and comprises a porcine circovirus genome, or a portion thereof capable of replication, and wherein said cell is permissive for the replication of said porcine circovirus.
A method of preparing a recombinant mammalian cell comprising a mammalian adenovirus E1 function and a porcine circovirus genome comprising the steps of, a) obtaining a mammalian cell expressing a mammalian adenovirus E1 function;
- and b) introducing said porcine circovirus genome, or a portion thereof capable of replication, into the mammalian cell.
Dependencies are inferred from the claim text.