Novel gene and method for producing L-amino acids
AssigneeAjinomoto Co., Inc.
InventorsVitaliy Livshits, Natalia Zakataeva, Vladimir Aleshin, Alla Belareva, Irina Tokhmakova
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Abstract
The present invention describes a bacterium which has an ability to produce an amino acid and in which the rhtC gene encoding a protein having an enhanced activity of imparting L-threonine resistance to a bacterium expressing the protein. Preferably, the bacterium further includes an rhtB gene encoding for a protein having an enhanced activity of imparting to a bacterium L-homoserine resistance expressing the protein. The present invention also describes a method of cultivating the bacterium in a culture medium to produce and accumulate amino acids in the medium, and the amino acid is recovered from the medium.
Claims7
A method for producing an amino acid comprising:
- A) cultivating in a culture medium an Escherichia bacterium which has been modified to increase expression of a DNA encoding a protein selected from the group consisting of
- a) a protein comprising the amino acid sequence of SEQ ID NO:4, and
- b) a protein comprising the amino acid sequence of SEQ ID NO:4, and which includes substitution, deletion, insertion, addition, or inversion of one or several amino acids in said sequence, wherein said protein is able to impart L-threonine resistance to said bacterium when expressed in said bacterium;
- and
- B) recovering said amino acid from the medium.
A method of producing an amino acids comprising:
- A) cultivating in a culture medium an Escherichia bacterium which has been modified to increase expression of a DNA selected from the group consisting of
- a) a DNA comprising the nucleotide sequence of numbers 187 to 804 in SEQ ID NO:3, and
- b) a DNA which is able hybridize with a nucleotide sequence of numbers 187 to 804 in SEQ ID NO:3 under stringent conditions, and encodes a protein which is able to impart L-threonine resistance to said bacterium when expressed in said bacterium;
- B) recovering said amino acid from the medium.
Dependencies are inferred from the claim text.