Method for producing L-amino acid using bacteria belonging to the genus Escherichia
AssigneeAjinomoto Co., Inc.
InventorsEkaterina Tabolina, Konstantin Rybak, Evgeni Khourges, Elvira Voroshilova, Mikhail Gusyatiner
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Abstract
There is provided a method for producing L-threonine, L-valine, L-proline, L-leucine, L-methionine and L-arginine using bacterium belonging to the genus Escherichia wherein L-amino acid productivity of the bacterium is enhanced by enhancing an activity of proteins coded by b2682 and b2683 genes, or protein coded by b1242 or b3434 gene.
Claims30
An L-amino acid producing bacterium belonging to the genus Escherichia, wherein the bacterium has been modified so that the L-amino acid production by said bacterium should be enhanced by enhancing activities of proteins as defined in the following (A) or (B), and (C) or (D) in a cell of said bacterium:
- (A) a protein which comprises the amino acid sequence shown in SEQ ID NO:3 in Sequence listing;
- (B) a protein which comprises an amino acid sequence including deletion, substitution, insertion or addition of one or several amino acids in the amino acid sequence shown in SEQ ID NO:3 in Sequence listing, and which has an activity of making bacterium having enhanced resistance to L-amino acids and/or its analogs;
- (C) a protein which comprises the amino acid sequence shown in SEQ ID NO:5 in Sequence listing;
- (D) a protein which comprises an amino acid sequence including deletion, substitution, insertion or addition of one or several amino acids in the amino acid sequence shown in SEQ ID NO:5 in Sequence listing, and which has an activity of making bacterium having enhanced resistance to L-amino acids and/or its analogs.
An L-amino acid producing bacterium belonging to the genus Escherichia, wherein the bacterium has been modified so that the L-amino acid production by said bacterium should be enhanced by enhancing activities of proteins as defined in the following (E) or (F) in a cell of said bacterium:
- (E) a protein which comprises the amino acid sequence shown in SEQ ID NO:11 in Sequence listing;
- (F) a protein which comprises an amino acid sequence including deletion, substitution, insertion or addition of one or several amino acids in the amino acid sequence shown in SEQ ID NO:11 in Sequence listing, and which has an activity of making bacterium having enhanced resistance to L-amino acids and/or its analogs;
An L-amino acid producing bacterium belonging to the genus Escherichia, wherein the bacterium has been modified so that the L-amino acid production by said bacterium should be enhanced by enhancing activities of proteins as defined in the following (G) or (H) in a cell of said bacterium:
- (G) a protein which comprises the amino acid sequence shown in SEQ ID NO:15 in Sequence listing;
- (H) a protein which comprises an amino acid sequence including deletion, substitution, insertion or addition of one or several amino acids in the amino acid sequence shown in SEQ ID NO:15 in Sequence listing, and which has an activity of making bacterium having enhanced resistance to L-amino acids and/or its analogs, such as DL-o-methylserine, 6-diazo-5-oxo-L-norleucine and DL-β-hydroxy-norvaline, and having enhanced sensitivity to S-(2-aminoethyl)cysteine
Dependencies are inferred from the claim text.