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US 2005/0239174 A1Published application
Enzymatic decarboxylation of 2-keto-L-gulonic acid to produce xylose
AssigneeArcher Daniels Midland Company
InventorsWuli Bao, Leif Solheim
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Abstract
The invention is drawn to the enzymatic decarboxylation of 2-keto-L-gulonic acid (2-KLG) to produce xylose. The invention is also drawn to a method to detect xylose in vitro or in vivo (intracellularly), which employs an L-xylose dehydrogenase.
Claims36
A method of producing xylose comprising enzymatically decarboxylating 2-keto-L-gulonic (2-KLG) acid to xylose.
A method of producing xylose in a cell free system comprising:
- a) converting 2-KLG to xylose in a mixture comprising pyruvate decarboxylase and 2-KLG;
- and
- b) purifying the xylose.
A method of producing xylose comprising:
- a) producing pyruvate decarboxylase using a host cell;
- b) producing 2-KLG from D-glucose or L-sorbose comprising:
- i) culturing a microorganism that produces 2-KLG from D-glucose or L-sorbose and
- ii) isolating the accumulated 2-KLG;
- and
- c) contacting the 2-KLG with the pyruvate decarboxylase to produce xylose.
3 more elements
A method to measure the production of xylose comprising:
- a) contacting L-xylose dehydrogenase with L-xylose in the presence of NAD+;
- and
- b) measuring NADH.
Dependencies are inferred from the claim text.