Production of amino sugars
AssigneeCargill, Incorporated
InventorsSara McFarlan, William Schroeder, Lawrence Fosdick, John Bohlmann
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Abstract
The present invention provides a method for producing an amino sugar selected from the group consisting of N-acetylglucosamine, glucosamine, or a combination thereof. The method comprises culturing a yeast in a culture medium and recovering N-acetylglucosamine, glucosamine, or a combination thereof, wherein the yeast comprises an exogenous nucleic acid sequence encoding glucosamine-6-phosphate synthase operably linked to a promoter. The invention also provides a genetically modified yeast that produces an amino sugar selected from the group consisting of N-acetylglucosamine, glucosamine, or a combination thereof.
Claims29
A method for producing an amino sugar in diploid yeast selected from the group consisting of N-acetylglucosamine, glucosamine, or a combination thereof comprising culturing a diploid yeast in a culture medium and recovering N-acetylglucosamine, glucosamine, or a combination thereof,
- wherein said yeast comprises an exogenous nucleic acid sequence encoding glucosamine-6-phosphate synthase operably linked to a promoter.
A method for producing glucosamine in diploid yeast comprising culturing a diploid yeast in a culture medium, performing deacetylation, and recovering glucosamine,
- wherein said yeast comprises an exogenous nucleic acid sequence encoding glucosamine-6-phosphate synthase operably linked to a promoter.
A method for producing an amino sugar in diploid yeast selected from the group consisting of N-acetylglucosamine, glucosamine, or a combination thereof comprising culturing a diploid yeast in a culture medium and recovering N-acetylglucosamine, glucosamine, or a combination thereof,
- wherein
- (a) said yeast comprises an exogenous nucleic acid sequence encoding glucosamine-6-phosphate synthase operably linked to a promoter, and
- (b) the pH of the culture medium is equal to or less than pH 5.0.
A genetically modified diploid yeast comprising
- (a) an exogenous nucleic acid sequence encoding glucosamine-6-phosphate synthase;
- and
- (b) one or more genetic modifications comprising disruption of a nucleic acid sequence encoding a peptide selected from the group consisting of 6-phosphofructo-2-kinase, phosphoglucomutase, phosphofructokinase alpha subunit, phosphofructokinase beta subunit, N-acetylglucosamine-6-phosphate mutase, UDP N-acetylglucosamine-6-phosphate pyrophosphorylase, glucosamine-6-phosphate N-acetyltransferase, mannose-6-phosphate isomerase, hexokinase I and chitin synthase.
Dependencies are inferred from the claim text.