Polynucleotides encoding human secreted protein, ZZP1
AssigneeZymoGenetics, Inc.
InventorsEdward Thayer, Philippa Webster
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Abstract
The present invention relates to polynucleotide and polypeptide molecules, and variants thereof, for Zzp1, a novel Zona Pellucida protein. The polypeptides, and polynucleotides encoding them, are fertility modulating and may be used for delivery and therapeutics. The present invention also includes antibodies to the Zzp1 polypeptides.
Claims20
An isolated polynucleotide encoding a polypeptide wherein the polypeptide comprises residues 26 to 546 of SEQ ID NO:2.
An isolated polynucleotide consisting of a nucleotide sequence that is complimentary to a polynucleotide selected from the group consisting of:
- (a) nucleotide 76 to 1638 of SEQ ID NO:1;
- (b) nucleotide 76 to 1881 of SEQ ID NO:1;
- (c) nucleotide 1 to 1638 of SEQ ID NO:1;
- (d) nucleotide 1 to 1881 of SEQ ID NO:1;
- and
- (e) nucleotide 1 to 1908 of SEQ ID NO:1.
A method of producing an antibody to a polypeptide comprising the following steps:
- inoculating an animal with the polypeptide such that the polypeptide elicits an immune response in the animal to produce the antibody;
- and
- isolating the antibody from the animal,
- wherein the polypeptide comprises at least fifteen consecutive amino acids of the amino acid sequence as shown in SEQ ID NO:2,
- and wherein the antibody produced by the method specifically binds to a polypeptide of SEQ ID NO:2.
A method for inhibiting sperm-oocyte fusion comprising contacting the sperm and oocytes with an isolated polypeptide comprising residues 26 to 546 of SEQ ID NO:2, whereby the polypeptide or fragment inhibits the sperm and oocyte fusion.
A method for inducing infertility in a mammal, comprising administering to the mammal an isolated polypeptide molecule comprising residues 26 to 546 of SEQ ID NO:2, wherein the polypeptide induces an immune response in the mammal, thereby inducing infertility in the mammal.
An isolated polypeptide molecule comprising residues 1 to 635 of SEQ ID NO:2.
Dependencies are inferred from the claim text.