Method for cultivation of the nitrile-hydratase-producing strain Rhodococcus rhodochrous M33
AssigneeStockhausen GmbH & Co. KG
InventorsJosef Lorenz, S.P. Woronin, S.W. Kosolin, I.N. Singirezev, V.G. Debabov, A.S. Yanenko
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Abstract
The invention relates to a method for cultivation of the nitrile-hydratase-producing strain Rhodococcus rhodochrous M33, using a culture medium which is based on a 12 to 60 mM phosphate buffer, covers the demand of the cells for phosphorus and maintains the pH in the range of 5.5 to 9.0 during cultivation, and to which acetic acid is added in doses as the new source of carbon after consumption of the initially supplied quantity of glucose. The invention also relates to the culture medium used in the method.
Claims25
A method for cultivating a nitrile-hydratase-producing Rhodococcus rhodochrous and/or for making nitrile-hydratase comprising:
- cultivating a nitrile-hydratase-producing strain of Rhodococcus rhodochrous in a medium containing a phosphate buffer and a first carbon source, and
- adding acetic acid and/or a salt(s) of acetic acid to the culture medium after depletion or consumption of the first carbon source and, optionally,
- isolating or recovering nitrile-hydratase.
A culture medium comprising:
- at least one phosphate buffer,
- 0.02 to 0.04 g/l of CoCl2×6 H2O,
- 1.0 g/l of MgSO4×7 H2O,
- 0 to 10 g/l of corn extract,
- 20 to 90 g/l of glucose,
- 4 to 20 g/l of urea, and optionally,
- 1 to 10 g/l corn extract and/or optionally acetic acid.
Dependencies are inferred from the claim text.