Glutathione production
AssigneeUnisearch Limited
InventorsGabriel Perrone, Ian Dawes, Christopher Grant
Life of this patent
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Abstract
The present invention relates to a process for the production of glutathione, wherein said process comprises culturing a mutant yeast strain under conditions promoting glutathione production, and wherein said yeast strain has one or more genetic mutations that result in increased secretion of glutathione into the culture medium relative to a parental strain.
Claims29
A process for the production of glutathione, wherein said process comprises culturing a mutant yeast strain under conditions promoting glutathione production, and wherein said yeast strain has one or more genetic mutations that result in increased secretion of glutathione into the culture medium relative to a parental strain and optionally isolating the glutathione from the culture medium.
A mutant yeast strain having at least two mutations selected from the group consisting of:
- i) mutation in a gene or genes encoding components of the mitochondrial respiratory chain or nuclear genes encoding proteins that maintain the integrity of the mitochondrial genome or mutation or deletion of the mitochondrial genome;
- ii) mutation in a gene or genes affecting intracellular levels of NAD(P)H and NAD(P)+;
- iii) mutation in a gene or genes affecting the assimilation and metabolism of nitrogen in the cell;
- iv) mutation in a gene or genes encoding regulatory components of the Ras/cAMP/PKA pathway or otherwise affecting the activity of the Ras/cAMP/PKA pathway;
- v) mutation in a gene or genes affecting endosomal function;
- vi) mutation in a gene or genes affecting the Golgi to endosome to vacuole transportation pathway or plasma membrane to endosome to vacuole traffic;
- vii) mutation in a gene or genes affecting ubiquitin levels and ubiquitin-mediated proteolysis via the 26S proteosome;
- viii) mutation in a gene or genes involved in transportation of glutathione across the yeast cell membrane;
- ix) mutation in a gene or genes involved in glutathione degradation;
- and
- x) mutation in a gene or genes involved in vacuolar function.
Dependencies are inferred from the claim text.