Method for detecting the von willebrand factor-cleaving protease activity of adamts-13
AssigneeDade Behring Marburg GmbH
InventorMartina Boehm
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Abstract
The invention relates to a diagnostic method for determining the von Willebrand factor (VWF) cleaving activity of ADAMTS-13 in a test medium during which the test medium is mixed with 0.5 to 5 U/ml of a von Willebrand factor (VWF) that does not contain ADAMTS-13, and after incubation, the ADAMTS-13 activity is determined based on the drop in the VWF-mediated aggregation of thrombocytes.
Claims25
A diagnostic method for determining the VWF-cleaving activity of ADAMTS-13 in a test medium, in which from 0.5 to 5 U of an ADAMTS-13-free von Willebrand factor (VWF), which has previously been incubated with urea, is/are added, per ml, to the test medium and, after incubation with the test medium, the ADAMTS-13 activity is determined by way of the reduction in the VWF-mediated aggregation of platelets.
A diagnostic method for determining the VWF-cleaving activity of ADAMTS-13 in a test medium, in which platelets are added to ADAMTS-13-free von Willebrand factor (VWF), with the platelets aggregating and the test medium then being added to this mixture and the ADAMTS-13 activity being determined by way of the dissociation of the platelet aggregates.
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A diagnostic kit, containing an ADAMTS-13-free VWF and platelets, as well as urea for pretreating the ADAMTS-13-free VWF.
Dependencies are inferred from the claim text.