Continuous-flow enzyme assay
AssigneeKiadis B.V.
InventorsHubertus Irth, Thomas Letzel
Life of this patent
Estimating the term…
Abstract
The present invention relates to an on-line detection method comprising the steps of: contacting an effluent of a fractionation step with a controlled amount of an enzyme; allowing the enzyme to interact with analytes suspected to be present in the effluent; addition of a controlled amount of a substrate for said enzyme; allowing a reaction of the enzyme with the substrate providing one or more modified substrate products; and detection of unreacted substrate, or a modified substrate product using a mass spectrometer.
Claims13
On-line detection method comprising the steps of:
- contacting an effluent of a fractionation step with a controlled amount of an enzyme;
- allowing the enzyme to interact with analytes suspected to be present in the effluent;
- addition of a controlled amount of a substate for said enzyme;
- allowing a reaction of the enzyme with the substrate providing one or more modified substrate products;
- and detection of unreacted substrate, or a modified substrate product using a mass spectrometer.
On-line detection method, wherein a mass spectrometer with a multiple-inlet unit is used, to which multiple-inlet unit different fractionation lines are connected, wherein each fractionation line comprises an effluent to which controlled amounts of enzyme and known substrates are added as described in each of the previous claims.
Dependencies are inferred from the claim text.