Method for enhancing staining of microorganisms
AssigneeVentana Medical Systems, Inc.
InventorsTimothy Fischer, Walter Sherman
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Abstract
The invention is directed to a method and composition of matter for enhancing the staining of tissue or features of interest therein by applying an effective amount of a bile salt in combination with a special stain to a tissue suspected of harboring a microorganism or other feature of diagnostic interest. The particular invention herein is directed to detecting H. pylori, the microorganism responsible for gastrointestinal ulcers. The composition of matter is a sensitizer for Alcian Yellow stain.
Claims11
A method of enhancing the staining of tissue or features of diagnostic interest therein by applying an effective amount of a bile salt in combination with a special stain to a tissue suspected of harboring a microorganism or other feature of diagnostic interest.
5-10. (canceled)
A method of enhancing the staining of tissue or features of diagnostic interest therein comprising applying an effective amount of sodium desoxycholate and Alcian Yellow to a tissue suspected of harboring a microorganism or other feature of diagnostic interest.
A method of enhancing the staining of gastric tissue or features of diagnostic interest therein comprising applying an effective amount of sodium desoxycholate and Alcian Yellow to gastric tissue suspected of harboring the H. pylori microorganism.
The method of claim 16 wherein siad sodium desoxycholate has a concentration range of from about 0.32% to about 0.64%.
Dependencies are inferred from the claim text.