Method for diagnosing infectious diseases
AssigneeUniversity of Tennessee Research Foundation
InventorsShigetoshi Eda, C. A. Speer
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Abstract
A method for diagnosing the presence in an animal of an infection due to a microorganism in which a test sample obtained from the animal is exposed to a population of the microorganism and the presence of binding of the microorganism by an antibody in the test sample is determined.
Claims22
A method for diagnosing the presence in an animal of an infection caused by a microorganism comprising obtaining a test sample from the animal, exposing the test sample to a population of the microorganism for a time sufficient to permit an antibody in the test sample that binds to an antibody binding site of the microorganism to bind to the antibody binding site, and determining if the test sample contains an antibody that binds to an antibody binding site of the microorganism by detecting the presence of a an antibody bound to an antibody binding site of the microorganism, wherein the microorganism is a mycobacterium.
The method of claim 3 wherein the mammal is a bovine.
(canceled)
The method of claim 21 wherein the detection of the complex is by blot analysis.
A method for diagnosing infection caused by Mycobacterium avium subsp. paratuberculosis (MAP) in an animal comprising obtaining a test sample from the animal, exposing the test sample to a population of MAP organisms for a time sufficient to permit an antibody in the test sample that binds to an antibody binding site of MAP to bind to the antibody binding site, and detecting the presence of binding of an antibody in the sample to the MAP organisms.
The method of claim 22 wherein the detection of the presence of binding is by blot analysis.
Dependencies are inferred from the claim text.