Flow-through membrane assays for carbohydrates using labeled lectins
OwnerAlbert Chuinventor
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Abstract
The present invention comprises a method for rapid detection of at least a first carbohydrate in a carbohydrate-containing sample molecule comprising the steps of: (a) retaining the sample molecule on a region of one liquid permeable reaction membrane, (b) flowing a solution of first lectin, capable of binding the first carbohydrate, through the one reaction membrane to bind the first lectin to the retained first carbohydrate, the lectin being directly conjugated to a label prior to binding to the carbohydrate or being bound to a labeled separate molecule only after the first lectin has been bound to the carbohydrate, and (c) thereafter, detecting the label bound on the one reaction membrane, indicating the presence of the first carbohydrate.
Claims10
A method for rapid detection of at least a first carbohydrate in a carbohydrate-containing sample molecule comprising the steps of:
- (a) retaining said sample molecule on a region of one liquid permeable reaction membrane,
- (b) flowing a solution first lectin, capable of binding said first carbohydrate, through said one reaction membrane to selectively bind said first lectin to said retained first carbohydrate, said lectin being directly conjugated to a label prior to binding to the carbohydrate or being bound to a label on a separate molecule after binding of said first lectin to said carbohydrate,
- (c) thereafter, detecting said label bound to said one reaction membrane, indicating the presence of said first carbohydrate.
Dependencies are inferred from the claim text.