Compositions, methods and kits for biarsenical fluorophore labeling
OwnerRoumen Bogoev, Joseph Amshey +1inventors
InventorsRoumen Bogoev, Joseph Amshey, George Hanson
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Abstract
Methods, compositions, and kits for labeling tetracysteine-tagged proteins with biarsenical fluorophores with increased specificity, including compositions, methods and kits particularly adapted for labeling of tetracysteine-tagged proteins to be resolved within an electrophoresis gel.
Claims48
A method of labeling a tetracysteine-tagged protein species present in a protein sample with a biarsenical fluorophore, the method comprising:
- treating the proteins in said sample in the presence of a composition comprising a thiol competitor and a biarsenical fluorophore.
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A composition for improving specificity of labeling of tetracysteine-tagged proteins with a biarsenical fluorophore, comprising:
- a biarsenical fluorophore;
- and
- a thiol competitor.
A kit for labeling tetracysteine-tagged proteins with a biarsenical fluorophore, comprising:
- a first composition comprising a thiol competitor.
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Dependencies are inferred from the claim text.