Method for identifying a modulator of the binding of CCX CKR polypeptide to a chemokine
AssigneeChemoCentryx, Inc.
InventorsJennifa Gosling, Daniel Dairaghi, Zhenhua Miao, Thomas Schall
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Abstract
The invention provides polypeptides and polynucleotides encoding a novel chemokine receptor, CCX CKR. The invention further provides reagents and methods for identifying agents that modulate the activity or expression of the receptors, as well as methods for detecting receptor expression.
Claims36
An isolated or recombinant CCX CKR polypeptide or fragment thereof, wherein said polypeptide or fragment binds ELC, SLC or TECK.
A polynucleotide comprising a sequence encoding a polypeptide that has a chemokine binding activity and which is:
- (a) a polynucleotide having the sequence of SEQ ID NO:1 or SEQ ID NO 3;
- or
- (b) a polynucleotide which hybridizes under stringent conditions to (a);
- or
- (c) a polynucleotide sequence which is degenerate as a result of the genetic code to the sequences defined in (a) or (b).
A polynucleotide primer, probe, antisense oligonucleotide or ribozyme comprising at least 15 contiguous bases identical or exactly complementary to SEQ ID NO:1.
A method of detecting an CCX CKR gene product in a sample comprising:
- a) contacting the sample with a probe that specifically binds the gene product, wherein the probe and the gene product form a complex, and detecting the formation of the complex;
- or,
- b) specifically amplifying the gene product in the biological sample, wherein said gene product is a polynucleotide, and detecting the amplification product;
- wherein the formation of the complex or presence of the amplification product is correlated with the presence of the CCX CKR gene product in the biological sample.
A method of amplifying a CCX CKR polynucleotide in a sample comprising
- (a) adding reagents sufficient for a polymerase chain reaction and at least two different primers to the sample, wherein each of said primers comprise at least 10 contiguous nucleotides identical or exactly complementary to SEQ. ID. NO:
- 1;
- or,
- (b) adding reagents sufficient for a ligase chain reaction and at least two different oligomers to the sample, wherein each of said primers comprise at least 10 contiguous nucleotides identical or exactly complementary to SEQ. ID. NO:
- 1.
A method for identifying a modulator of the binding of CCX CKR to a chemokine comprising
- (a) contacting an isolated or recombinant CCX CKR polypeptide and the chemokine in the presence of a test compound, and
- (b) comparing the level of binding of the chemokine and the polypeptide in (a) with the level of binding in the absence of the test compound,
- wherein a decrease in binding indicates that the test compound is an inhibitor of binding and an increase in binding indicates that the test compound is an enhancer of binding.
A method of treating an CCX CKR-mediated condition in a mammal comprising administering an agent that modulates the activity or expression of CCX CKR in a cell or tissue in the mammal.
Dependencies are inferred from the claim text.