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OwnerHugh Brady, Catherine Godson +3inventors
InventorsHugh Brady, Catherine Godson, Finian Martin, Ruth McMahon, Madeline Murphy
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A method for identifying a gene having a role in the presentation of diabetic nephropathy comprises culturing mesangial cells in the presence of a concentration of glucose sufficient to induce differential expression, especially up-regulation, of a gene susceptible to such differential expression and identifying the gene so induced. The cells are also optionally subjected to mechanical strain and/or TGF-β1 can be added to the culture medium. The differentially expressed genes can be identified by suppression subtractive hybridisation. The method has resulted in the identification of novel genes which play a role in the presentation of diabetic nephropathy. The genes can be used as diagnostic markers for diabetic nephropathy and as the basis of drug development programmes.
A method of diagnosing and determining the progression of diabetic nephropathy which comprises, culturing mesangial cells in a medium in the presence of transforming growth factor β1 (TGF-β1) and a concentration of glucose sufficient to induce differential expression of a gene susceptible to such differential expression;
A method of identifying drugs for use in the prevention and/or therapy of diabetic nephropathy which comprises, culturing mesangial cells in a medium in the presence of transforming growth factor β1 (TGF-β1) and a concentration of glucose sufficient to induce differential expression of a gene susceptible to such differential expression;
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