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US 2005/0239125 A1Published application
Methods for genotype screening
OwnerTimothy Hodgeinventor
Life of this patent
Estimating the term…
Abstract
The present invention provides a method to rapidly provide genotype screening of a plurality of biological samples in a designated well of a microwell container for remote user by a screening laboratory. The screening method can be used to determine if a biological sample is heterozygous, homozygous or wild for a designated genetic sequence.
Claims50
A method to screen a biological sample for at least one designated genetic sequence within 24 hours of receiving the biological sample at a screening laboratory:
- a) acquiring the identity of at least one designated genetic sequence for a plurality of samples;
- b) acquiring positive and negative controls for said designated genetic sequence;
- c) obtaining means to determine the presence of said designated genetic sequence;
- d) receiving said biological sample from a remote user at a screening laboratory;
- e) reporting screening result to the remote user within 24 hours of receiving said sample at said screening laboratory.
2 more elements
A method for detecting at least one designated genetic sequence in a biological sample comprising:
- a) treating said biological sample to obtain a lysate containing cellular debris including a genomic nucleic acid, wherein genomic said nucleic acid includes at least a portion of an intact nucleic acid;
- b) separating a standard concentration of genomic nucleic acid using magnetic particles;
- and
- c) screening said standard concentration of genomic nucleic acid to detect said designated genetic sequence.
1 more element
A method to report screening results to a remote user by a screening laboratory for a plurality of samples including genomic nucleic acid comprising:
- (a) acquiring the identity of at least one designated genetic sequence for each of said plurality of samples;
- (b) receiving, at a screening laboratory, the plurality of samples, wherein each of said plurality of samples has been deposited in a designated well of a source well container;
- (c) treating each of the said plurality of samples to obtain a lysate containing cellular debris including genomic nucleic acid, wherein said genomic nucleic acid includes at least a portion of intact genomic nucleic acid;
- (d) separating a standard concentration of genomic nucleic acids wherein the step of separating a standard concentration of genomic nucleic acid includes the step of treating said lysate to saturate magnetic particles with genomic nucleic acid;
- (e) screening said standard concentration of genomic nucleic acid to obtain screening results;
- and
- (f) reporting said screening results to said remote user.
4 more elements
A method to identify homozygous, heterozygous and wild type samples for at least one designated genetic sequence comprising:
- (a) receiving, at a screening laboratory a plurality of samples, wherein each of said plurality of samples has been deposited in a designated well of a source well container by a remote user;
- (b) receiving screening parameter selections that identify at least one designated genetic sequence;
- (c) treating each of said at plurality samples to obtain a lysate containing cellular debris including genomic nucleic acid wherein said genomic nucleic acid includes at least a portion of intact genomic nucleic acid;
- (d) separating a standard concentration of genomic nucleic acid using magnetic particles;
- (e) screening said standard concentration of genomic nucleic acid to obtain screening results;
- (f) reporting said screening results to said remote user said screening results including signal magnitude for at least one designated genetic sequence for each of said plurality of samples;
- and
- (g) receiving a designation of signal magnitude corresponding to a sample selected from the group consisting of homozygous, heterozygous and wild type sample from said remote user.
5 more elements
A method for detecting at least one designated genetic sequence in a biological sample comprising:
- a) treating said biological sample to obtain a lysate containing cellular debris including a genomic nucleic acid, wherein said genomic nucleic acid includes at least a portion of intact nucleic acid;
- b) separating said genomic nucleic acid using magnetic particles;
- c) adding at least one probe and primer set corresponding to one of said at least one designated genetic sequence to said biological sample in said at least one well of a microwell container;
- d) adding at least one probe and primer set corresponding to a reference sequence to said biological sample in said at least one well of a microwell container;
- e) screening said biological sample in said at least one well of a microwell container to obtain screening results, wherein one of said screening results is probe values;
- and
- f) comparing the probe values for said probe corresponding to said designated genetic sequence with said probe value corresponding to said reference sequence to detect said at least one designated genetic sequence.
4 more elements
A method for evaluating the validity of data obtained from genotype screening of a strain including at least one designated genetic sequence comprising:
- (a) dispensing an aliquot of a biological sample of the strain into at least two wells of a microwell container;
- (b) adding at least one probe and primer set corresponding to at least one designated genetic sequence to said biological sample in one of said at least two wells of said microwell container;
- (c) adding a probe and primer set corresponding to a reference sequence to the other of one of said at least two wells of said microwell container;
- (d) screening said biological sample in said at least two wells of said microwell container to obtain screening results;
- (e) comparing the screening results between said at least two wells of said microwell container to evaluate the validity of data obtained from genotype screening.
2 more elements
A method for evaluating the validity of data obtained from genotype screening of a strain including at least one designated genetic sequence.
- (a) dispensing an aliquot of a biological sample of the strain into at least one well of a microwell container;
- (b) adding at least one probe and primer set corresponding to a designated genetic sequence to said biological sample and at least one probe and primer set corresponding to a reference sequence to said at least one well of a microwell container;
- (c) screening said biological sample in said at least one well of said microwell container to obtain screening results wherein in one of the screening results is probe values;
- (d) comparing the probe values for said probe corresponding to said designated genetic sequence with said probe value corresponding to said reference sequence to detect said at least one designated genetic sequence.
1 more element
A method to obtain purified human genomic nucleic acid from a sample using magnetic particles comprising:
- (a) treating said sample to obtain a lysate containing cellular debris including at least a portion of intact genomic nucleic acid;
- (b) treating said lysate to bind said genomic nucleic acid to said magnetic particles;
- (c) separating said genomic nucleic acid using magnetic particles;
- and
- (d) disassociating said genomic nucleic acid from said magnetic particles to obtain said purified human genomic nucleic acid including at least a portion of intact genomic nucleic acid.
2 more elements
Dependencies are inferred from the claim text.