Nucleic acid sequences that can be used as primers and probes in the amplification and detection of all subtypes of HIV-1

A pair of oligonucleotide primers, consisting of a first oligonucleotide being 15-50 nucleotides in length and comprising at least a fragment of 15 sequential nucleotides of a sequence selected from the group consisting of:

• G GGC GCC ACT GCT AGA GA;:
• SEQ ID NO:1
• G TTC GGG CGC CAC TGC TAG A;:
• SEQ ID NO:2
• and
• CGG GCG CCA CTG CTA,:
• SEQ ID NO:3
• and a second oligonucleotide being 15-50 nucleotides in length and comprising at least a fragment of 15 sequential nucleotides of a sequence selected from the group consisting of:
• CTG CTT AAA GCC TCA ATA AA;:
• SEQ ID NO:4
• and
• CTC AAT AAA GCT TGC CTT GA.:
• SEQ ID NO:5

The pair of oligonucleotides of claim 1, consisting of a first oligonucleotide being 15-50 nucleotides in length and comprising at least a fragment of 15 sequential nucleotides of the sequence:

• SEQ ID NO:1:
• G GGC GCC ACT GCT AGA GA;
• and a second oligonucleotide being 15-50 nucleotides in length and comprising at least a fragment of 15 sequential nucleotides of the sequence SEQ ID NO:5:
• CTC AAT AAA GCT TGC CTT GA.

A pair of hybridizing oligonucleotide primers, consisting of a first hybridizing oligonucleotide being 15-50 nucleotides in length and comprising at least a fragment of 15 sequential nucleotides of a hybridizing sequence selected from the group consisting of:

• G GGC GCC ACT GCT AGA GA;:
• SEQ ID NO:1
• G TTC GGG CGC CAC TGC TAG A;:
• SEQ ID NO:2
• and
• CGG GCG CCA CTG CTA;:
• SEQ ID NO:3
• and a second hybridizing oligonucleotide being 15-50 nucleotides in length and comprising at least a fragment of 15 sequential nucleotides of a hybridizing sequence selected from the group consisting of:
• CTG CTT AAA GCC TCA ATA AA;:
• SEQ ID NO:4
• and
• CTC AAT AAA GCT TGC CTT GA.:
• SEQ ID NO:5

A pair of oligonucleotide primers, consisting of a first oligonucleotide being 10-50 nucleotides in length and comprising at least a fragment of 10 sequential nucleotides of a sequence selected from the group consisting of:

• G GGC GCC ACT GCT AGA GA;
• SEQ ID NO:1:
• G TTC GGG CGC CAC TGC TAG A;
• SEQ ID NO:2:
• and
• CGG GCG CCA CTG CTA,
• SEQ ID NO:3:
• wherein said first oligonucleotide is operably linked to a promoter sequence;
• and
• a second oligonucleotide being 10-50 nucleotides in length and comprising at least a fragment of 10 sequential nucleotides of a sequence selected from the group consisting of:
• CTG CTT AAA GCC TCA ATA AA;
• SEQ ID NO:4:
• and
• CTC AAT AAA GCT TGC CTT GA.
• SEQ ID NO:5:

The pair of oligonucleotide primers of claim 24, consisting of a first oligonucleotide being 10-50 nucleotides in length and comprising at least a fragment of 10 sequential nucleotides of the sequence:

• SEQ ID NO:1:
• G GGC GCC ACT GCT AGA GA;
• and a second oligonucleotide being 10-50 nucleotides in length and comprising at least a fragment of 10 sequential nucleotides of the sequence SEQ ID NO:5:
• CTC AAT AAA GCT TGC CUT GA.

A primer set consisting of:

• a) a first oligonucleotide being 10-50 nucleotides in length and comprising at least a fragment of 10 sequential nucleotides of a sequence selected from the group consisting of:
• G GGC GCC ACT GCT AGA GA;
• SEQ ID NO:1:
• G TTC GGG CGC CAC TGG TAG A;
• SEQ ID NO:2:
• and
• CGG GCG CCA CTG CTA;
• SEQ ID NO:3:
• b) a second oligonucleotide being 10-50 nucleotides in length and comprising at least a fragment of 10 sequential nucleotides of a sequence selected from the group consisting of:
• CTG CTT AAA CCC TCA ATA AA;
• SEQ ID NQ:4:
• and
• CTC AAT AAA GCT TGC CTT GA;
• SEQ ID NO:5:
• and
• c) a promoter sequence.

The primer set of claim 29, consisting of a first oligonucleotide being 10-50 nucleotides in length and comprising at least a fragment of 10 sequential nucleotides of the sequence:

• SEQ ID NO:1:
• G GGC GCC ACT GCT AGA GA;
• a second oligonucleotide being 10-50 nucleotides in length and comprising at least a fragment of 10 sequential nucleotides of the sequence SEQ ID NO:5:
• CTC AAT AAA GCT TGC CTT GA;
• and a T7 promoter sequence operably linked to said first oligonucleotide sequence.

A pair of oligonucleotide primers, consisting of a first oligonucleotide being 15-50 nucleotides in length and comprising at least a fragment of 15 sequential nucleotides of a sequence selected from the group consisting of:

• G GGC GCC ACT GCT AGA GA;
• SEQ ID NO:1:
• G TTC GGG CGC CAC TGC TAG A;
• SEQ ID NO:2:
• CGG GCG CCA CTG CTA;
• SEQ ID NO:3:
• SEQ ID NO:9:
• aat tct aat acg act cac tat agg gAG AGG GGC GCC ACT GCT AGA GA;
• and
• a second oligonucleotide being 15-50 nucleotides in length and comprising at least a fragment of 15 sequential nucleotides of a sequence selected from the group consisting of:
• CTG CTT AAA TCC TCA ATA AA;
• SEQ ID NO:4:
• and
• CTC AAT AAA GCT TGC CTT GA.
• SEQ ID NO:5:

The pair of oligonucleotide primers of claim 32, consisting of a first oligonucleotide being 15-50 nucleotides in length and comprising at least a fragment of 15 sequential nucleotides of the sequence:

• SEQ ID NO:9:
• aat tct aat acg act cac tat agg gAG AGO GGC GCC ACT GCT AGA GA;
• and a second oligonucleotide being 15-50 nucleotides in length and comprising at least a fragment of 15 sequential nucleotides of the sequence SEQ ID NO:5:
• CTC AAT AAA GCT TGC CTT GA.

A pair of oligonucleotide primers, consisting of a first oligonucleotide being 10-50 nucleotides in length and comprising a non-hybridizing promoter region and a hybridizing region, wherein said hybridizing region comprises at least a fragment of 10 sequential nucleotides of a sequence selected from the group consisting of:

• G GGC GCC ACT GCT AGA GA;
• SEQ ID NO:1:
• G TTC GGG CGC CAC TGC TAG A;
• SEQ ID NO:2:
• CGG GCG CCA CTG CTA;
• SEQ ID NO:3:
• and
• a second oligonucleotide being 10-50 nucleotides in length and comprising a hybridizing region that comprises at least a fragment of 10 sequential nucleotides of a sequence selected from the group consisting of:
• CTG CTT AAA GCC TCA ATA AA;
• SEQ ID NO:4:
• and
• CTC AAT AAA GCT TGC CTT GA.
• SEQ ID NO:5: