Back to results
US 2005/0239116 A1Published application
Methods and compositions for assaying analytes
OwnerJames Willeyinventor
Life of this patent
Estimating the term…
Abstract
The present invention is directed to methods and compositions for assaying one or more analytes in a sample with improved sensitivity, reproducibility and/or lower detection limits, as well as applications employing such compositions and methods. In particular, the present invention provides methods and compositions for assaying one or more non-nucleic acid analytes with one or more other nucleic acid and/or non-nucleic acid analytes that serve as controls.
Claims25
A method for assaying an analyte in a sample, comprising:
- allowing said analyte to couple to a first nucleic acid;
- mixing said analyte with a known amount of an antagonist for said analyte wherein said antagonist is coupled to a competitive template for said first nucleic acid;
- exposing said mixture to a receptor that can form complexes with both said antagonist and said analyte;
- obtaining a first relation, said first relation comparing amplified product of said first nucleic acid that complexed with said receptor to co-amplified product of said competitive template for said first nucleic acid that complexed with said receptor;
- mixing a reference nucleic acid in said first sample with a known amount of a competitive template for said reference nucleic acid wherein said reference nucleic acid is a control for loading;
- obtaining a second relation, said second relation comparing amplified product of said reference nucleic acid to co-amplified product of said competitive template for said reference nucleic acid;
- and
- comparing said first and said second relations, thereby assaying said analyte.
5 more elements
A method for assessing a first analyte in a sample, comprising:
- allowing said first analyte to couple to a first nucleic acid;
- allowing a second analyte in said sample to couple to a second nucleic acid;
- mixing said first and said second analytes with known amounts of an antagonist for said first analyte coupled to a competitive template for said first nucleic acid and an antagonist for said second analyte coupled to a competitive template for said second nucleic acid;
- exposing said mixture to a first receptor that can form complexes with said antagonist for said first analyte and with said first analyte and to a second receptor that can form complexes with said antagonist for said second analyte and with said second analyte;
- obtaining a first relation, said first relation comparing amplified product of said first nucleic acid that complexed with said first receptor to co-amplified product of said competitive template for said first nucleic acid that complexed with said first receptor;
- obtaining a second relation, said second relation comparing amplified product of said second nucleic acid that complexed with said second receptor to co-amplified product of said competitive template for said second nucleic acid that complexed with said second receptor;
- and
- comparing said first and said second relations;
- thereby assessing an amount of said first analyte.
6 more elements
A method for assessing an analyte in a sample, comprising:
- allowing said analyte to couple to a first nucleic acid;
- mixing said analyte with a known amount of an antagonist for said analyte wherein said antagonist is coupled to a competitive template for said first nucleic acid;
- exposing said mixture to a receptor that can form complexes with both said antagonist and said analyte;
- co-amplifying said first nucleic acid that complexed with said receptor and said competitive template for said first nucleic acid that complexed with said receptor;
- and
- obtaining a relation wherein said relation is substantially constant beyond an exponential phase of said co-amplification;
- thereby assessing an amount of said analyte.
4 more elements
Dependencies are inferred from the claim text.