Methods and devices for microarray image analysis
AssigneeAffymetrix, Inc.
InventorsJekwan Ryu, Richard Rava, Stephen Fodor
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Abstract
The present invention provides methods and devices for high sensitivity and high speed microarray optical imaging. The methods include using patterned excitation to obtain a series of images and analyzing the images to resolve probe intensities which reflect the hybridization or binding between target and probes. Probe feature information and patterned excitation (structured illumination) information are incorporated into the analysis.
Claims27
A computer readable medium comprising computer-executable instructions for performing the method comprising:
- Obtaining a series of fluorescent images of a microarray, wherein the fluorescent signals reflect binding between targets and probes, and wherein each of the images is obtained with a different excitation pattern;
- and
- Analyzing said images using calibrated information about said different excitation patterns to obtain intensities for each of said probes.
A computer readable medium comprising computer-executable instructions for performing the method comprising:
- Obtaining a series of fluorescent images of a microarray, wherein the fluorescent signals reflect binding between targets and probes, and wherein each of the images is obtained with a different excitation pattern;
- and
- Analyzing said images using calibrated information about said different excitation patterns and probe feature information to obtain intensities for each of said probes.
Dependencies are inferred from the claim text.