US 2005/0239098 A1 · Novel hyaluronan-binding proteins and encoding genes

Abstract

The present invention relates to full-length WF-HABP, WF-HABP, OE-HABP, and BM-HABP, novel members of the hyaluronan receptor family. The invention provides isolated nucleic acid molecules encoding human to full-length WF-HABP, WF-HABP, OE-HABP, and BM-HABP receptors. Full-length WF-HABP, WF-HABP, OE-HABP, and BM-HABP polypeptides are also provided, as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of full-length WF-HABP, WF-HABP, OE-HABP, and BM-HABP receptor activity. Also provided are diagnostic methods for detecting disease states related to the aberrant expression of full-length WF-HABP, WF-HABP, OE-HABP, and BM-HABP receptors. Further provided are therapeutic methods for treating disease states including, but not limited to, proliferative conditions, metastasis, inflammation, ischemia, host defense dysfunction, immune surveillance dysfunction, arthritis, multiple sclerosis, autoimmunity, immune dysfunction, and allergy.

An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:

(a) a polynucleotide fragment of SEQ ID NO:
1;
(b) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:2;
(c) a polynucleotide encoding a polypeptide domain of SEQ ID NO:2;
(d) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:2;
(e) a polynucleotide encoding a polypeptide of SEQ ID NO:2 having biological activity;
and
(f) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-(e), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues. (g) a polynucleotide fragment of SEQ ID NO:4;
(h) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:5 or a polypeptide encoded by the cDNA sequence included in ATCC Deposit No:
203503;
(i) a polynucleotide encoding a polypeptide domain of SEQ ID NO:5 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
203503;
(j) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:5 or, a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
203503;
(k) a polynucleotide encoding a polypeptide of SEQ ID NO:5 or a polypeptide encoded by the cDNA sequence included in ATCC Deposit No:
203503, having biological activity;
and
(l) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (g)-(k), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.
(m) a polynucleotide fragment of SEQ ID NO:7;
(n) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:8 or a polypeptide encoded by the cDNA sequence included in ATCC Deposit No:
203501;
(o) a polynucleotide encoding a polypeptide domain of SEQ ID NO:8 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
203501;
(p) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:8 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
203501;
(q) a polynucleotide encoding a polypeptide of SEQ ID NO:8 or a polypeptide encoded by the cDNA sequence included in ATCC Deposit No:
203501, having biological activity;
and
(r) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (m)-(q), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.
(s) a polynucleotide fragment of SEQ ID NO:
10;
(t) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:11 or a polypeptide encoded by the cDNA sequence included in ATCC Deposit No:
203502;
(u) a polynucleotide encoding a polypeptide domain of SEQ ID NO:
11 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
203502;
(v) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:
11 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
203502;
(w) a polynucleotide encoding a polypeptide of SEQ ID NO:
11 or a polypeptide encoded by the cDNA sequence included in ATCC Deposit No:
203502, having biological activity;
and
(x) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (s)-(w), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.

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Novel hyaluronan-binding proteins and encoding genes

Life of this patent

Novel hyaluronan-binding proteins and encoding genes

Life of this patent

Abstract

Claims23