Device for sequential protein transfer from a gel

A method for sequentially receiving molecules from a separation medium for use in a separation method comprising the steps of:

• receiving a first substantially separated molecule in a first receptacle of an apparatus comprising a separation medium having an outlet and a collector having at least the first receptacle and a second receptacle wherein the first receptacle and the second receptacle can be sequentially brought into contact with the outlet of the separation medium by translating the first receptacle and the second receptacle in relation to the outlet of the separation medium;
• translating the first receptacle and the second receptacle such that the second receptacle is brought into in contact with the outlet of the separation medium;
• receiving a second substantially separated molecule in the second receptacle;
• and
• repeating said steps to sequentially receive a desired number of substantially separated molecules.

A method for sequentially electroeluting biomolecules from an electrophoretic separation medium comprising the steps of:

• receiving a first substantially separated biomolecule in a first slot of an apparatus comprising a separation gel having an outlet and a collection drum having at least the first slot and a second slot wherein the first slot and the second slot can be sequentially brought into contact with the outlet of the separation gel by translating the first slot and the second slot in relation to the outlet of the separation gel and wherein the first slot and the second slot comprising a slot gel;
• translating the first slot and the second slot such that the second slot is brought into in contact with the outlet of the separation gel;
• receiving a second substantially separated biomolecule in the second slot;
• repeating said steps to sequentially receive a desired number of substantially separated biomolecules;
• and
• analyzing at least one of the substantially separated biomolecules.

A kit for conducting electrophoretic separation of biomolecules comprising:

• a separation medium having an outlet and an inlet;
• a collector comprising at least a first receptacle and a second receptacle that can be sequentially brought into contact with the outlet of the separation medium by translating the first receptacle and the second receptacle in relation to the outlet of the separation medium;
• an anode;
• and
• a cathode.