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US 2005/0239085 A1Published application
Methods for nucleic acid sequence determination
OwnerPhilip Buzby, Rebecca Ickes +1inventors
InventorsPhilip Buzby, Rebecca Ickes, James DiMeo
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Abstract
Methods of the invention comprise methods for nucleic acid sequence determination. Generally, the invention relates to sequencing a target nucleic acid by exposing the target nucleic acid to a primer and a polymerase. Such methods may involve determining the sequence of a target nucleic acid by using a thermophilic polymerase, such as a variant of said 9° N DNA polymerase.
Claims40
A method for nucleic acid sequence determination, the method comprising the steps of:
- (a) exposing a target nucleic acid to a primer that is complementary to at least a portion of the target, a thermophilic polymerase, and at least one nucleotide for extension of said primer;
- (b) conducting a primer extension at a temperature of about 20-70° C.;
- (c) detecting incorporation of said nucleotide in said primer;
- and,
- (d) repeating steps (a), (b) and (c), thereby to determine a sequence of said target.
2 more elements
Dependencies are inferred from the claim text.