Sequence tag microarray and method for detection of multiple proteins through DNA methods
AssigneeLARGE SCALE PROTEOMICS CORPORATION, A Division of Large Scale Biology Corporation
InventorN. Anderson
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Abstract
Methods and reagents for simultaneously measuring the concentration of numerous proteins in a sample are described. The technique uses many antibody display phages that contain corresponding specific nucleic acid sequence tags. Binding between various proteins and antibodies is determined by simultaneous detection of the specific sequence tags using a microarray. This method is applicable even when the concentrations of different proteins differ by orders of magnitude as the nucleic acid sequence tags may be amplified.
Claims39
A microarray having immobilized thereon a plurality of oligonucleotides complementary to sequence tags.
A recombinant microorganism capable of expressing a specific receptor on its surface and containing a unique nucleic acid sequence tag.
A nucleic acid labeled receptor comprising;
- a specific binding receptor, and
- a nucleic acid containing at least 13 nucleotides,
- wherein the nucleic acid is physically or chemically bound to the specific binding receptor.
A microarray comprising;
- a solid phase containing a plurality of cells in a definable location,
- a plurality of nucleic acids immobilized on the solid phase, wherein each cell of the solid phase contains all of the nucleic acids of a particular sequence, and
- a nucleic acid sequence tag specifically hybridized to the nucleic acid.
A microarray comprising;
- a solid phase containing a plurality of cells in a definable location,
- a plurality of nucleic acids immobilized on the solid phase, wherein each cell of the solid phase contains all of the nucleic acids of a particular sequence and wherein a nucleic acid sequence for each of the nucleic acids is complementary to predefined sequence tags, each with a different nucleotide sequence.
A solid support having bound thereto a plurality of different recombinant microorganisms capable of expressing a specific receptor on its surface wherein the recombinant microorganism contains a heterologous gene encoding the receptor.
A method for fractionating a mixture of recombinant microorganisms, each capable of expressing a different specific receptor on a surface thereof comprising;
- contacting the mixture with a solid support and allowing at least part of the mixture to become bound thereto,
- removing unbound recombinant microorganisms.
Dependencies are inferred from the claim text.