Human lung adenocarcinoma-related monoclonal antibody and antigen and immunaossay method which uses the same
AssigneeFujirebio Inc.
InventorsKouichi Tsuji, Yoshihiro Yoshida, Takashi Uwano
Life of this patent
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Abstract
The monoclonal antibody produced by a hybridoma cell strain TRD-L1, -L2 or -L3 which is obtained by the fusion between mouse myeloma cells and spleen cells of a mouse immunized with a human lung adenocarcinoma cell secretion component reacts in a specific fashion with a glycoprotein antigen that has a molecular weight of 200 kD or more (SDS-PAGE) and is present in human lung adenocarcinoma cells. It can be used efficiently in cancer diagnosis.
Claims12
1-9. (canceled)
A monoclonal antibody or antigen binding fragment binding to a glycoprotein antigen which is produced from a human pulmonary adenocarcinoma cell and has a molecular weight of 200 kD or more determined by SDS-PAGE, the glycoprotein antigen reacting with PNA lectin and not reacting with GNA lectin, SNA lectin and DSA lectin.
The monoclonal antibody or antigen binding fragment according to claim 1, wherein a sugar chain terminal structure of the glycoprotein antigen has Gal (1-3) GalNAc structure and does not have Mannose, NeuAc (2-6) Gal and Gal (1-4) GlcNAc structures.
The monoclonal antibody or antigen binding fragment according to claim 1, wherein the antibody or fragment is of IgM isotype.
The monoclonal antibody or antigen binding fragment according to claim 1, wherein the antibody or fragment is a fragment selected from the group of a Fab, Fab(ab)2 and a Fv fragment.
The monoclonal antibody or antigen binding fragment according to claim 1, wherein the antibody or fragment directly or indirectly is attached to a detectable label.
An immunoassay method for diagnosis of human pulmonary adenocarcinoma,
- the method comprising:
- contacting a sample of a subject with a monoclonal antibody or antigen binding fragment binding to a glycoprotein antigen which is produced from the human pulmonary adenocarcinoma cell and has a molecular weight of 200 kD or more determined by SDS-PAGE, the glycoprotein antigen reacting with PNA lectin and not reacting with GNA lectin, SNA lectin and DSA lectin;
- and
- measuring binding amount obtained on the basis of the contacting between the antibody or fragment and the glycoprotein antigen in the sample.
The immunoassay method according to claim 6, wherein a sugar chain terminal structure of the glycoprotein antigen has Gal (1-3) GalNAc structure and does not have Mannose, NeuAc (2-6) Gal and Gal (1-4) GlcNAc structures.
The immunoassay method according to claim 6, wherein the sample is human serum or human tissue.
The immunoassay method according to claim 6, wherein the antibody or fragment directly or indirectly is attached to a detectable label.
The immunoassay method according to claim 6, wherein the immunoassay method is a sandwich enzyme assay method.
The immunoassay method according to claim 9, wherein the label is an enzyme.
Dependencies are inferred from the claim text.