Device and method for measuring glycosaminoglycans in body fluids
OwnerSteven Striepeke, Zhiqi Gouilloninventors
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Abstract
The present invention features automated devices, kits, and assay methods for measuring glycosaminoglycan levels in body fluids such as urinary glycosaminoglycan excretion or blood heparin levels. Such devices, kits and methods are useful, e.g., for monitoring heparin therapy or diagnosing or monitoring mucopolysaccharidoses.
Claims57
A glycosaminoglycan measuring device, comprising:
- a sample entry port adapted to accept a bodily fluid sample;
- a glycosaminoglycan separation cartridge coupled to the sample entry port and adapted to separate glycosaminoglycans from interfering substances in the sample;
- and
- a detection apparatus comprising a detection chamber coupled to the glycosaminoglycan separation cartridge and adapted to detect the separated glycosaminoglycans.
A glycosaminoglycan measuring device comprising:
- a sample entry port coupled to a first transport line;
- a first reagent container coupled to a second transport line;
- a second reagent container coupled to a third transport line;
- a glycosaminoglycan separation cartridge coupled to one or more transport lines;
- a waste discharging port coupled to a fourth transport line;
- a detecting reagent container coupled to a fifth transport line;
- a pump operably connected to one or more of the transport lines;
- and
- a detection apparatus operably connected to the device.
A glycosaminoglycan measuring device comprising a sample entry port, separator means for substantially separating glycosaminoglycans from a sample delivered at the sample entry port to produce separated glycosaminoglycans, and detection means for detecting the separated glycosaminoglycans.
A method of measuring glycosaminoglycans in a body fluid sample, comprising the steps of:
- (a) automatically delivering a portion of the sample to a glycosaminoglycan separation cartridge;
- (b) separating glycosaminoglycans from interfering substances using the glycosaminoglycan separation cartridge;
- (c) automatically delivering the separated glycosaminoglycans to a detection apparatus;
- (d) combining a detection reagent with the separated glycosaminoglycans;
- and
- (e) detecting the amount of separated glycosaminoglycans by detecting the amount of detection reagent bound to the separated glycosaminoglycans.
Dependencies are inferred from the claim text.