Materials and methods to produce stem cells
OwnerSteve Oh, Andre Chooinventors
Life of this patent
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Abstract
A method of generating stem cells, such as human and mouse embryonic stem cells, from a stem cell culture comprising the step of perfusing the stem cell culture by flowing culture media through the stem cell culture and apparatus for generating stem cells are disclosed. Also disclosed are methods and apparatus for generating stem cells, such as human and mouse embryonic cells, by culturing the cells on a plurality of hydrophilic or porous membranes. Further disclosed is a supplemented conditioned medium for obtaining improved yields of stem cells, such as human and mouse embryonic stem cells.
Claims17
An apparatus for generating stem cells from a stem cell culture by perfusing the stem cells with culture media, the apparatus comprising:
- a plurality of membranes configured for attachment of one or more stem cells to be cultured;
- culture media supply means for supplying culture media to the stem cell culture;
- culture media removal means for removing culture media from said stem cell culture,
- wherein said supply and removal means are arranged to flow culture media through the stem cell culture.
An apparatus for culturing stem cells comprising:
- (a) a housing having a wall defining an internal chamber;
- (b) a plurality of stem cell culture membrane extending across said chamber and held in position by;
- (b) membrane support means;
- and further comprising
- (c) means to supply culture media to said membranes.
Dependencies are inferred from the claim text.