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US 2005/0233441 A1Published application
Cell culture device
AssigneeInstitut National de la Recherche Agronomique
InventorJean-Pierre Ozil
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AssigneeInstitut National de la Recherche Agronomique
InventorJean-Pierre Ozil
Estimating the term…
The invention relates to a device that is intended, in particular, for the culture of cells. The inventive device consists of a chamber (100) which is intended to receive the cells (10) and which comprises at least one liquid injection conduit (121, 122, 123, 124, 125, 126), the surface of the liquid contained in said chamber (100) being in free contact with the air. The invention is characterised in that it comprises a collector element (40) which can form at least one liquid bridge with the free surface of the liquid, said collector element (40) comprising a mouth piece (43) which is positioned essentially at the level of the free surface of the liquid contained in the chamber (100). The aforementioned mouth piece (43) is maintained at a vacuum such that it absorbs a flow of air which moves the surface film (30) of the liquid via the liquid bridge.
A device intended in particular for culturing cells, comprising a chamber (100) intended to receive the cells (10), said chamber (100) comprising at least one liquid injection conduit (121, 122, 123, 124, 125, 126), the surface of the liquid contained in the chamber (100) being in free contact with the air, characterized in that it comprises a collector element (40) which can form at least one liquid bridge (51, 52) with the free surface of the liquid, the collector element (40) comprising a mouthpiece (42) which is placed substantially at the level of the free surface of the liquid contained in the chamber (100), the mouthpiece (42) being kept at low pressure such that it absorbs a flow of air which moves the surface film (30) of the liquid via the liquid bridge (51, 52).
The device as claimed in any one of the preceding claims, characterized in that mouthpiece (42) of the collector element (40) is elongated in shape and extends longitudinally along the edge of the wall (11).
The device as claimed in one of the preceding claims, characterized in that the mouthpiece (42) has a generally rectangular shape.
The device as claimed in one of the preceding claims, characterized in that it comprises several collector elements (40) placed along the edges of the walls (11, 12, 13) of the chamber (100).
A method for regulating the level of a liquid contained in a chamber (100) intended to receive cells (10), said chamber (100) comprising at least one liquid injection conduit (121, 122, 123, 124, 125, 126), the surface of the liquid contained in the chamber being in free contact with the air, characterized in that at least one liquid bridge (51, 52) is formed with the free surface of the liquid and a flow of air is drawn by suction in the vicinity of the liquid bridge (51, 52), such that this flow of air moves the surface film (30) of the liquid via the liquid bridge (51, 52).
Dependencies are inferred from the claim text.