Cytokine polypeptides
AssigneeImmunex Corporation
InventorsPeter Baum, Bruce Mosley
Life of this patent
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Abstract
This invention relates to TMEM7 and TMEM7-related cytokines and to new members of the human cytokine polypeptide family, to methods of making such polypeptides, to methods of using them to treat conditions and diseases involving proliferation and/or differentiation of cells from pluripotent stem cell precursors, and to methods of using them to identify compounds that alter cytokine polypeptide activities.
Claims23
An isolated polypeptide consisting essentially of an amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence that begins between amino acid 3 and amino acid 8 of SEQ ID NO:2, and ends between amino acid 119 and amino acid 136 of SEQ ID NO:2;
- (b) a fragment of an amino acid sequence of (a) having cytokine polypeptide activity;
- (c) an amino acid sequence of any of (a) or (b) further comprising a CEAC motif (amino acids 154 through 157 of SEQ ID NO:2) amino acid sequence;
- wherein said isolated polypeptide does not consist of the amino acid sequence of any of the polypeptides disclosed in Kiss et al., 2002, Eur J Hum Genet 10:
- 52-61;
- GenBank NM—031440.1, GenBank XP—087464, and EP 1 104 808 A1.
An isolated polypeptide consisting essentially of the amino acid sequence of amino acid 3 of SEQ ID NO:2 through amino acid 136 of SEQ ID NO:2;
- wherein said isolated polypeptide does not consist of the amino acid sequence of any of the polypeptides disclosed in Kiss et al., 2002, Eur J Hum Genet 10:
- 52-61;
- GenBank NM—031440.1, GenBank XP—087464, and EP 1 104 808 A1.
An isolated polypeptide consisting essentially of an amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence that begins between amino acid 3 through amino acid 8 of SEQ ID NO:2 and ends between amino acid 119 through amino acid 136 of SEQ ID NO:2;
- (b) a fragment of an amino acid sequence of (a) having cytokine polypeptide activity;
- (c) an amino acid sequence of any of (a) or (b) further comprising a CEAC motif (amino acids 154 through 157 of SEQ ID NO:2) amino acid sequence;
- (d) amino acid sequences comprising at least 20 amino acids and sharing amino acid identity with the amino acid sequences of any of (a)-(b), wherein the percent amino acid identity is selected from the group consisting of:
- at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97.5%, at least 99%, and at least 99.5%;
- (e) an amino acid sequence of (d), wherein a polypeptide comprising said amino acid sequence of (d) binds to an antibody that also binds to a polypeptide comprising an amino acid sequence of any of (a)-(c);
- and
- (f) an amino acid sequence of (d) or (e) having cytokine polypeptide activity;
- wherein said isolated polypeptide does not consist of the amino acid sequence of any of the polypeptides disclosed in Kiss et al., 2002, Eur J Hum Genet 10:
- 52-61;
- GenBank NM—031440.1, GenBank XP—087464, and EP 1 104 808 A1.
An isolated polypeptide consisting essentially of an amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence that begins between amino acid A through B and ends between amino acid Y through Z, wherein sets of values for A, B, Y, and Z are selected from the group consisting of:
- A=3, B=8, Y=19, and Z=34 of SEQ ID NO:2;
- A=56, B=64, Y=71, and Z=76 of SEQ ID NO:2;
- A=77, B=86, Y=92, and Z=92 of SEQ ID NO:2;
- and A=100, B=108, Y=119, and Z=136 of SEQ NO:2;
- (b) a fragment of an amino acid sequence of (a) comprising at least 20 contiguous amino acids;
- (c) a fragment of an amino acid sequence of (a) comprising at least 30 contiguous amino acids;
- (d) a fragment of an amino acid sequence of (a) having cytoline polypeptide activity;
- (e) a fragment of an amino acid sequence of any of (a) comprising Helix A and/or Helix D and/or further comprising CEAC motif (amino acids 154 through 157 of SEQ ID NO:2) amino acid sequences;
- (f) amino acid sequences comprising at least 20 amino acids and sharing amino acid identity with the amino acid sequences of any of (a)-(e), wherein the percent amino acid identity is selected from the group consisting of:
- at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97.5%, at least 99%, and at least 99.5%;
- (g) an amino acid sequence of (f), wherein a polypeptide comprising said amino acid sequence of (f) binds to an antibody that also binds to a polypeptide comprising an amino acid sequence of any of (a)-(e);
- and
- (h) an amino acid sequence of (f) or (g) having cytokine polypeptide activity;
- wherein said isolated polypeptide does not consist of the amino acid sequence of any of the polypeptides disclosed in Kiss et al., 2002, Eur J Hum Genet 10:
- 52-61;
- GenBank NM—031440.1, GenBank XP—087464, and EP 1 104 808 A1.
Dependencies are inferred from the claim text.