Detection of antibodies to gangliosides using solid-phase reactants coated with carbonyl groups
AssigneeAthena Diagnostics, Inc.
InventorsNormand Robichaud, Louis Kertiles
Life of this patent
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Abstract
Methods of detecting antibodies to one or more ganglioside(s) of interest in a sample are disclosed which comprise using a solid-phase reactant having carbonyl groups attached thereon, and the ganglioside(s) of interest linked to the solid-phase reactant by an amide bond between an amino group of the ganglioside of interest and a carbonyl group attached to the solid-phase reactant. The methods of detecting antibodies to ganglioside(s) of interest can be used in methods of diagnosing neuropathies in an individual.
Claims11
A method of diagnosing a neuropathy in an individual, comprising determining the amount of antibody to a ganglioside of interest in a test sample from the individual by conducting an assay using a modified solid-phase reactant having carbonyl groups attached thereon and a ganglioside of interest linked to the modified solid-phase reactant by an amide bond between an amino group of the ganglioside of interest and a carbonyl group attached to the modified solid-phase reactant, wherein an amount of antibody to a ganglioside of interest that is greater, by an amount that is statistically significant, than the amount in a control sample, is indicative of the presence of the neuropathy.
A method of diagnosing a neuropathy in an individual, comprising determining the amount of antibody to a ganglioside of interest in a test sample from the individual by conducting an assay using a modified solid-phase reactant having carbonyl groups attached thereon and a ganglioside of interest linked to the modified solid-phase reactant by an amide bond between an amino group of the ganglioside of interest and a carbonyl group attached to the modified solid-phase reactant, wherein an amount of antibody to a ganglioside of interest that is equal to or greater than a reference amount is indicative of the presence of the neuropathy.
A kit for detecting the amount of antibody to a ganglioside of interest, comprising a modified solid-phase reactant having carbonyl groups attached thereon and a ganglioside of interest linked to the modified solid-phase reactant by an amide bond between an amino group of the ganglioside of interest and a carbonyl group attached to the modified solid-phase reactant.
Dependencies are inferred from the claim text.