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US 2005/0214919 A1Published application
Novel lipase genes
AssigneeCodexis, Inc.
InventorsLorraine Giver, Jeremy Minshull, Kurt Vogel
Life of this patent
Estimating the term…
Abstract
New lipase enzymes (both nucleic acids and polypeptides) are provided. Compositions which include these polypeptides, proteins, nucleic acids, recombinant cells, as well as methods involving the enzymes, antibodies to the enzymes, and methods of using the enzymes are also provided.
Claims41
1-180. (canceled)
An isolated or recombinant nucleic acid encoding a polypeptide having lipase activity and comprising a sequence having at least 94% amino acid sequence identity to the mature region of SEQ ID NO:
- 55.
An isolated or recombinant nucleic acid encoding a polypeptide encoded having lipase activity,
- wherein the nucleic acid hybridizes under stringent conditions over substantially the entire length of SEQ ID NO:
- 1, or complement thereof,
- wherein stringent hybridization conditions are 42° C. overnight in 50% formamide with 1 mg heparin and a 0.2×SSC wash at 65° C. for 15 minutes, and
- wherein the polypeptide comprises one or more amino acid residues selected from the group consisting of Lys at position 1, Thr at position 14, Ser at position 17, Arg at position 22, Glu at position 26, Pro at position 31, Gly at position 33, Glu at position 34, Pro at position 35, Pro or Thur at position 37, Ser or Lys at position 41, Gly at position 42, Arg or Glu at position 43, Ala at position 61, Tyr at position 75, Gly at position 96, Ser at position 97, Thr at position 104, Ser at position 107, Ala at position 125, Gly at position 129, Val at position 134, Cys at position 138, Lys at position 141, Lys at position 146, Thr at position 156, Met at position 160, Arg at position 166, and His at position 177,
- wherein the positions are equivalent amino acid positions with respect to SEQ ID NO:
- 75.
3 more elements
Dependencies are inferred from the claim text.