Somatic cell line
OwnerYann Echelard, Esmail Behboodi +3inventors
InventorsYann Echelard, Esmail Behboodi, David Melican, Carol Ziomek, William Gavin
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Abstract
The invention features a purified embryonic or fetal caprine somatic cell. The somatic cell can be in a preparation of embryonic or fetal caprine somatic cells. In addition, the cell can be used to derive an embryonic or fetal caprine somatic cell line. The somatic cell can be a genetically engineered caprine somatic cell, e.g., a somatic cell which includes a transgenic sequence, or which a nucleic acid has been introduced.
Claims40
1-91. (canceled)
A method for the accelerated production of transgenic animals comprising:
- a) transfecting a first non-human differentiated somatic cell or cell-line with a transgene construct containing a first DNA sequence;
- b) selecting a transfected cell or cell-line into which said first DNA sequence has been inserted into the genome of said first non-human differentiated somatic cell or cell-line;
- c) performing a first nuclear transfer procedure to generate a first transgenic animal at least heterozygous for said first DNA sequence;
- d) performing a biopsy or other cell selection technique to obtain cells to establish a second non-human differentiated somatic cell or cell-line from said first transgenic animal;
- e) characterizing said second non-human differentiated somatic cell or cell-line using known molecular biology methods to ensure that the selected said second non-human differentiated somatic cell or cell-line is at least heterozygous for said first DNA sequence;
- and
- f) performing a second nuclear transfer procedure with at least one of said second non-human differentiated somatic cells to produce at least a second transgenic animal at least heterozygous for said first DNA sequence.
A method of preparing a genetically engineered transgenic mammal, comprising:
- (a) inseminating a first female non-human mammal recipient with semen from a transgenic non-human animal of the same species known to have a transgene present and expressed;
- (b) obtaining a transgenic non-human embryo from said first female recipient;
- (c) obtaining a somatic cell from said embryo;
- (d) culturing said differentiated somatic cell in a suitable medium, such that a differentiated somatic cell line is obtained and,
- (e) performing a nuclear transfer procedure with said non-human differentiated somatic cells to produce at least one transgenic mammal at least heterozygous for said first DNA sequence;
- wherein said first DNA sequence encoding a desired gene is actuated by a tissue specific promoter.
Dependencies are inferred from the claim text.