Carboxypeptidases and nucleic acids encoding same
AssigneesNovozymes A/S, Novozymes Biotech, Inc.
InventorsAlexander Blinkovsky, Randy Berka, Michael Rey, Elizabeth Golightly, Alan Klotz, Thomas Mathisen, Claus Dambmann
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Abstract
The present invention relates to polypeptides having carboxypeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to methods of obtaining protein hydrolysates useful as flavor improving agents.
Claims27
A method of producing a hydrolysate from a proteinaceous substrate which comprises subjecting the substrate to an endopeptidase and a polypeptide having carboxypeptidase activity, wherein the polypeptide having carboxypeptidase activity is selected from the group consisting of:
- (a) a polypeptide having an amino acid sequence which has at least 95% identity with amino acids 19 to 555 of SEQ ID NO:
- 2;
- (b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under medium stringency conditions nucleotides 55 to 1662 of SEQ ID NO:
- 1, or its complementary strand, wherein medium stringency conditions are defined as prehybridization and hybridization a 42° C. in 5×SSPE, 0.3% SDS, 200 mg/ml sheared and denatured salmon sperm DNA, and 35% formamide;
- and
- (c) a fragment of (a) or (b), wherein the fragment has carboxypeptidase activity.
A method of obtaining from a proteinaceous substrate a hydrolysate enriched in free glutamic acid and/or peptide bound glutamic acid residues, comprising subjecting the substrate to a deamidation process and a polypeptide having carboxypeptidase activity selected from the group consisting of:
- (a) a polypeptide having an amino acid sequence which has at least 95% identity with amino acids 19 to 555 of SEQ ID NO:
- 2;
- (b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under medium stringency conditions nucleotides 55 to 1662 of SEQ ID NO:
- 1, or its complementary strand, wherein medium stringency conditions are defined as prehybridization and hybridization a 42° C. in 5×SSPE, 0.3% SDS, 200 mg/ml sheared and denatured salmon sperm DNA, and 35% formamide;
- and
- (c) a fragment of (a) or (b), wherein the fragment has carboxypeptidase activity.
Dependencies are inferred from the claim text.