Monoclonal antibody recognizing phosphatidylinositol-3,4,5-triphosphate
AssigneesMedical & Biological Laboratories Co., Ltd., Yasuhisa Fukui
InventorsYasuhisa Fukui, Satoshi Nagata, Ryuichi Shirai
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Abstract
A novel monoclonal antibody that specifically recognizes phosphatidylinositol-3,4,5-triphosphate (PIP3) but does not cross-react with structurally similar phospholipid antigens is advantageous for PIP3-specific immunoassay. The gene in the variable regions of the monoclonal antibody has been identified, which enables producing recombinant antibodies.
Claims5
1-17. (canceled)
An immunoassay kit for assaying phosphatidylinositol-3,4,5-triphosphate, wherein said kit comprises a monoclonal antibody, or a variable region thereof, which:
- (a) specifically recognizes phosphatidylinositol-3,4,5-triphosphate;
- and;
- (b) binds to an antigenic determinant that contains an inositol group and a glycerol backbone in said phosphatidylinositol-3,4,5-triphosphate.
An immunoassay kit for assaying phosphatidylinositol-3,4,5-triphosphate, wherein the kit comprises a monoclonal antibody specifically recognizing Phosphatidylinositol-3,4,5-triphosphate, or a variable region thereof, wherein said monoclonal antibody comprises an immunoglobulin, or variable region thereof, which recognizes phosphatidylinositol-3,4,5-triphosphate, wherein said immunoglobulin, or variable region thereof, comprises an immunoglobulin heavy chain comprising:
- (i) CDR1 comprising the amino acid sequence of SEQ ID NO:
- 5;
- (ii) CDR2 comprising the amino acid sequence of SEQ ID NO:
- 6;
- and,
- (iii) CDR3 comprising the amino acid sequence of SEQ ID NO:
- 7.
Dependencies are inferred from the claim text.