Methods for cloning animals
AssigneeTrustees of Tufts College
InventorsAlexander Baguisi, Eric W. Overstrom
Life of this patent
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Abstract
The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an activated enucleated oocyte to thereby obtain a nuclear transfer embryo, and impregnating an animal with the nuclear transfer embryo in conditions suitable for gestation of a cloned animal. The invention further relates to methods of chemically enucleating an oocyte having a meiotic spindle apparatus by exposing the oocyte with a compound that destabilizes the meiotic spindle apparatus.
Claims9
1-30. (canceled)
A method of enucleating an oocyte having a meiotic spindle apparatus, comprising exposing the oocyte with at least one compound that destabilizes the meiotic spindle apparatus.
A method of preparing an oocyte for nuclear transfer, wherein the oocyte has a meiotic spindle apparatus, comprising the steps of:
- a. exposing the oocyte to ethanol, ionophore, and/or to electrical stimulation, to thereby obtain an activated oocyte, and
- b. subjecting the activated oocyte to at least one compound that destabilizes the meiotic spindle apparatus, to thereby enucleate the activated oocyte.
Dependencies are inferred from the claim text.