Novel treatment for pathologies associated with oxidative damage
OwnerTianyun Wu, William McIntireinventors
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Abstract
This invention relates to compositions and methods for the treatment of pathologies associated with intracellular polyamine dysregulation. In particular, the present invention provides compositions and methods involving mammalian polyamine oxidase (PAO) to treat cancer, cell damage, tissue damage caused by ischemia/reperfusion, inflammation, traumatic brain injury, stroke and tissue developmental disorders. Methods for diagnosis and prognosis of cancer and other diseases are also provided by the present invention.
Claims30
An isolated nucleic acid that comprises a sequence selected from the group consisting of a gene encoding a peroxisomal acetylpolyamine oxidase protein of a mammal, a gene encoding a biologically active portion of said peroxisomal acetylpolyamine oxidase protein, and a gene encoding a biologically active variant of said peroxisomal acetylpolyamine oxidase protein.
An isolated mammalian nucleic acid sequence selected from the group consisting of the open reading frames of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 and sequences that hybridize to the complement of the open reading frames of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, under conditions of low stringency, and wherein said isolated nucleic acid sequence encodes a polypeptide having amine oxidizing activity.
A composition comprising an isolated protein selected from the group consisting of a peroxisomal acetylpolyamine oxidase of a mammal, a biologically active portion thereof, or a biologically active variant thereof.
A method for detecting mammalian peroxisomal acetylpolyamine oxidase expression in a cell comprising the steps of:
- a) providing:
- i) a sample from a mammalian subject, and
- ii) at least one reagent capable of specifically detecting mammalian peroxisomal acetylpolyamine oxidase expression;
- and
- b) contacting said sample with said at least one reagent under conditions suitable for binding said at least one reagent to a mammalian peroxisomal acetylpolyamine oxidase gene product.
A method of inhibiting mammalian peroxisomal acetylpolyamine oxidase activity comprising:
- a) providing a mammalian peroxisomal acetylpolyamine oxidase, and an inhibitor;
- and
- b) contacting said mammalian peroxisomal acetylpolyamine oxidase with said inhibitor under conditions suitable for reducing amine oxidizing activity of said oxidase.
A method comprising:
- a) providing a host cell comprising an exogenous nucleic sequence selected from the group consisting of the open reading frames of SEQ ID NO:1, SEQ NO:3, SEQ D NO:5 and sequences that hybridize to the complement of the open reading frames of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 under conditions of low stringency, wherein said isolated nucleic acid sequence encodes a polypeptide having amine oxidizing activity;
- and
- b) culturing said host cell under conditions such that said exogenous nucleic acid sequence is expressed.
Dependencies are inferred from the claim text.