Novel genetic products of ashbya gossypii, associated with the mechanisms of signal transduction and especially with the improvement of vitamin b2 production
OwnerMarvin Karos, Henning Althofer +2inventors
InventorsMarvin Karos, Henning Althofer, Burkhard Kroger, Jose Revuelta Doval
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Abstract
The invention relates to novel polynucleotides from Ashbya gossypii; to oligonucleotides hybridizing therewith; to expression cassettes and vectors which comprise these polynucleotides; to microorganisms transformed therewith; to polypeptides encoded by these polynucleotides; and to the use of the novel polypeptides and polynucleotides as targets for improving signal transduction mechanisms and, in particular, improving vitamin B2 production in microorganisms of the genus Ashbya.
Claims38
An isolated polynucleotide comprising a sequence that codes for a protein associated with signal transduction activity that can be isolated from a microorganism of Ashbya gossypii or a functional equivalent of said protein.
A recombinant prokaryotic or eukaryotic host cell that possesses a signal transduction activity wherein functional expression of a gene that codes for a polypeptide associated with said signal transduction activity is modulated;
- or a biological activity of a said polypeptide is reduced or increased.
A method for detecting an effector target for modulating the microbiological production of vitamin B2 or a precursor or derivative thereof, comprising:
- treating a microorganism capable of the microbiological production of vitamin B2 or a precursor or derivative thereof is treated with an effector that interacts with a polypeptide associated with signal transduction activity that can be isolated from a microorganism of Ashbya gossypii or a nucleic acid sequence that encodes said polypeptide, and
- determining an effect of the effector on the amount of the microbiologically produced vitamin B2 or a precursor or derivative thereof.
A method for modulating the microbiological production of vitamin B2 or a precursor or derivative thereof, comprising:
- treating a microorganism capable of the microbiological production of vitamin B2 or a precursor or derivative thereof with an effector that interacts with a polypeptide associated with signal transduction activity that can be isolated from a microorganism of Ashbya gossypii or a nucleic acid sequence that encodes said polypeptide.
An isolated effector for a polypeptide associated with signal transduction activity that can be isolated from a microorganism of Ashbya gossypii or a nucleic acid sequence that encodes said polypeptide.
A polynucleotide encoded by at least ten consecutive amino acid residues of the sequence of SEQ ID NOs:
- 2, 4, 6, or 8, or a functional equivalent of said at least ten consecutive amino acid residues.
Dependencies are inferred from the claim text.