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OwnerNicolas Escriou, Sylvie Van Der Werf +2inventors
InventorsNicolas Escriou, Sylvie Van Der Werf, Marco Vignuzzi, Sylvie Gerbaud
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The present invention relates to replicons or self-replicating RNA molecules, derived from the genome of cardioviruses and aphtoviruses, which can be used to express heterologous proteins in animal cells. When injected in an animal host, for example in the form of naked RNA, these replicons permit the translation of the encoded heterologous protein. If the encoded heterologous protein is a foreign antigen, these replicons induce an immune response against the encoded heterologous protein. The invention uses cardiovirus and aphtovirus genomes to construct these replicons. The invention demonstrates that these replicons, when injected as naked RNA, can induce immune responses against a replicon-encoded heterologous protein in an animal recipient without the help of any kind of carrier or adjuvant.
A self-replicating recombinant positive strand RNA molecule of a viral genome of an RNA virus, wherein the RNA molecule comprises:
A self-replicating recombinant positive strand RNA molecule of a viral genome of an RNA virus, wherein the RNA molecule comprises:
A DNA molecule that encodes a self-replicating recombinant positive strand RNA molecule of a viral genome of an RNA virus, wherein the RNA molecule comprises:
A DNA molecule that encodes a self-replicating recombinant positive strand RNA molecule of a viral genome of an RNA virus, wherein the RNA molecule comprises:
A DNA molecule comprising the sequence of SEQ. ID. NO. 26 (deposited at CNCM, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France, on May 21, 2001, under Accession No.1-2668) or a fragment thereof, and DNA sequence encoding a heterologous protein or fragment of a heterologous protein in an expressible form.
A DNA molecule comprising the sequence of SEQ. ID. NO. 26 (deposited at CNCM, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France, on May 21, 2001, under Accession No. I-2668) either in a mutated or truncated form or a fragment thereof and DNA sequence encoding a heterologous protein or fragment of a heterologous protein in an expressible form.
A DNA molecule comprising the sequence of SEQ. ID. NO. 27 (deposited at the CNCM, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France, on May 21, 2001, under Accession No. I-2669) or a fragment thereof and DNA sequence encoding a heterologous protein or fragment of a heterologous protein.
A DNA molecule comprising the sequence of SEQ. ID. NO. 27 (deposited at the CNCM, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France, on May 21, 2001, under Accession No. I-2669) either in a mutated or truncated form or a fragment thereof and DNA sequence encoding a heterologous protein or fragment of a heterologous protein in an expressible form.
A DNA molecule, comprising the sequence of SEQ. ID. NO. 28 (deposited at CNCM, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France, on May 16, 2002, under Accession No. I-2879).
Dependencies are inferred from the claim text.