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AssigneeThe Regents of the University of California
InventorsRaul Andino-Pavlovsky, Marco Vignuzzi, Derek Wells
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The present invention provides a live, attenuated, invasive bacterium that infects a mammalian host cell, and releases exogenous RNA into the cytoplasm of the host cell. The present invention further provides compositions, including immunogenic compositions, comprising a subject bacterium. The present invention provides methods of delivering an RNA to a eukaryotic host cell in vitro, ex vivo, or in vivo. The present invention provides methods of delivering a protein to a host cell in vitro, ex vivo, or in vivo. The present invention provides methods of controlling expression of a target gene in a eukaryotic host cell. The present invention provides methods of inducing an immune response in a mammalian host to a polypeptide antigen, the method involving administering to the host a subject bacterium, wherein the antigen is encoded by the exogenous RNA produced by the bacterium.
A bacterial delivery system comprising a live, attenuated bacterium comprising a DNA expression vector, which DNA expression vector comprises a nucleotide sequence encoding an exogenous RNA, wherein the nucleotide sequence is operably linked to a heterologous promoter that is functional in the bacterium, wherein said bacterium synthesizes the exogenous RNA encoded by the DNA vector, and wherein, following infection of a eukaryotic host cell, the bacterium releases the exogenous RNA into the cytoplasm of the eukaryotic host cell.
Dependencies are inferred from the claim text.