Mammalian cell lines specifically deficient in O-linked glycosylation
OwnerJudith Fridovich-Keil, Monty Krieger +3inventors
InventorsJudith Fridovich-Keil, Monty Krieger, Hazel Holden, James Thoden, Jenny Schulz
Life of this patent
Estimating the term…
Abstract
Genetically modified cell lines that express a UDP-galactose 4-epimerase (GALE) capable of interconverting UDP-galactose (UDP-gal) and UDP-glucose (UDP-glc), but essentially incapable of interconverting UDP-N-acetylgalactosamine (UDP-galNAc) and UDP-N-acetylglucosamine (UDP-glcNAc).
Claims28
An isolated polynucleotide comprising a polynucleotide selected from:
- a polynucleotide sequence set forth in SEQ ID NO:
- 1 (C307YhGALE) or a degenerate variant of the SEQ ID NO:
- 1;
- a polynucleotide sequence at least 90% identical to the polynucleotide sequence set forth in SEQ ID NO:
- 1;
- a polynucleotide sequence at least 75% identical to the polynucleotide sequence set forth in SEQ ID NO:
- 1;
- and a polynucleotide sequence at least 50% identical to the polynucleotide sequence set forth in SEQ ID NO:
- 1.
A polypeptide selected from:
- an amino acid sequence set forth in SEQ ID NO:
- 2 (C307YhGALE), or conservatively modified variants thereof;
- an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 2;
- an amino acid sequence that is at least 75% identical to SEQ ID NO:
- 2;
- and an amino acid sequence that is at least 50% identical to SEQ ID NO:
- 2.
A cell line transfected with an expression vector comprising a polynucleotide selected from:
- a polynucleotide sequence set forth in SEQ ID NO:
- 1 (C307YhGALE) or a degenerate variant of the SEQ ID NO:
- 1;
- a polynucleotide sequence at least 90% identical to the polynucleotide sequence set forth in SEQ ID NO:
- 1;
- a polynucleotide sequence at least 75% identical to the polynucleotide sequence set forth in SEQ ID NO:
- 1;
- and a polynucleotide sequence at least 50% identical to the polynucleotide sequence set forth in SEQ ID NO:
- 1, encoding a polypeptide having the characteristics that the polypeptide is capable of UDP-gal/UDP-glc interconversion and substantially incapable of UDP-galNAc/UDP-glcNAc interconversion.
A vector comprising an isolated polynucleotide selected from:
- a polynucleotide sequence set forth in SEQ ID NO:
- 3 (WTeGALE), or a degenerate variant of the SEQ ID NO:
- 3;
- a polynucleotide sequence at least 90% identical to the polynucleotide sequence set forth in SEQ ID NO:
- 3;
- a polynucleotide sequence at least 75% identical to the polynucleotide sequence set forth in SEQ ID NO:
- 3;
- and a polynucleotide sequence at least 50% identical to the polynucleotide sequence set forth in SEQ ID NO:
- 3.
A cell line transfected with an expression vector comprising a polynucleotide selected from:
- a polynucleotide SEQ ID NO:
- 3 (WTeGALE) or a degenerate variant of the SEQ ID NO:
- 3;
- a polynucleotide sequence at least 90% identical to the polynucleotide sequence set forth in SEQ ID NO:
- 3;
- a polynucleotide sequence at least 75% identical to the polynucleotide sequence set forth in SEQ ID NO:
- 3;
- and a polynucleotide sequence at least 50% identical to the polynucleotide sequence set forth in SEQ ID NO:
- 3 encoding a polypeptide having the characteristics that the polypeptide is capable of UDP-gal/UDP-glc interconversion and substantially incapable of UDP-galNAc/UDP-glcNAc interconversion.
Dependencies are inferred from the claim text.