Cytidine deaminase activators, deoxycytidine deaminase activators, Vif antagonists, and methods of screening for molecules thereof
OwnerHarold Smith, Joseph Wedekind +3inventors
InventorsHarold Smith, Joseph Wedekind, Mark Sowden, Stephen Dewhurst, Baek Kim
Life of this patent
Estimating the term…
Abstract
Disclosed are compounds that enhance RNA or DNA editing, as well as methods of using, identifying, and making such compounds. These compounds include Vif antagonists and cytidine deaminase inhibitors.
Claims62
A method of screening for a Vif antagonist, comprising:
- (a) contacting a Vif molecule with a test compound;
- (b) detecting binding between the Vif molecule and the test compound;
- and
- (c) screening the test compound that binds the Vif molecule for suppression of viral infectivity, suppression of viral infectivity by the test compound indicating the test compound is a Vif antagonist.
A method of screening for cytidine deaminase activators, comprising:
- (a) contacting a cytidine deaminase molecule with a test compound;
- (b) detecting binding between the cytidine deaminase molecule and the test compound;
- (c) screening the test compound that binds the cytidine deaminase molecule to identify a selected cytidine deaminase function, the presence of the selected function indicating a cytidine deaminase activator.
A polypeptide comprising 5 or more contiguous amino acid residues of a ubiquitination protein, wherein the polypeptide binds Vif and blocks ubiquitination of CEM15.
A polypeptide comprising 5 or more contiguous amino acid residues of a Gag protein, wherein the polypeptide binds CEM15 and promotes CEM15 binding to viral RNA.
A polypeptide comprising 5 or more contiguous amino acid residues of CEM15 wherien the polypeptide binds a ubiquitination protein and blocks Vif-mediated ubiquitination of CEM15.
A polypeptide comprising 5 or more contiguous amino acid residues of a CEM15 binding domain on Vif, wherein the polypeptide blocks CEM15-Vif interaction.
Dependencies are inferred from the claim text.