Mammalian CDP-diacylglycerol synthase
AssigneeCell Therapeutics, Inc.
InventorDavid Leung
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Abstract
There is disclosed cDNA sequences and polypeptides having the enzyme CDP-diacylglycerol synthase (CDS) activity. CDS is also known as CTP:phosphatidate cytidylyltransferase. There is further disclosed methods for isolation and production of polypeptides involved in phosphatidic acid metabolism and signaling in mammalian cells, in particular, the production of purified forms of CDS.
Claims9
1-16. (canceled)
An isolated polynucleotide, comprising a polynucleotide sequence selected from the group consisting of:
- (a) the DNA sequence of SEQ ID NO. 11, (b) a DNA sequence, which encodes the polypeptide of SEQ ID NO. 12 and any biologically active fragments thereof, (c) a DNA sequence that has at least 85% sequence identity to the sequence depicted in SEQ ID NO. 11, and (d) a DNA sequence that has at least 85% sequence identity to a DNA sequence that encodes the polypeptide of SEQ ID NO. 12 or any biologically active fragments thereof, wherein said isolated polynucleotide encodes a polypeptide having cytidine diphosphate diacylglycerol synthase (CDS) activity.
An isolated polynucleotide comprising a polynucleotide sequence that hybridizes under high stringency conditions of either (i) incubation in 5×SSC at 65° C., followed by washing in 0.1×SSC for 30 minutes, or (ii) incubation in 50% formamide and 5×SSC at 42° C. to the complement of the DNA sequence depicted in SEQ ID NO. 11, wherein said polynucleotide encodes a protein having CDS activity.
Dependencies are inferred from the claim text.