Transformation method for obtaining marker-free plants and plants obtained therewith
OwnerAnna Wolters, Richard Gerardus Visser +3inventors
InventorsAnna Wolters, Richard Gerardus Visser, Ingrid van der Meer, Paul Heeres, Nicolaas Clemens de Vetten
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Abstract
The invention relates to a transformation method for obtaining transgenic plants and plants obtained with said method. The invention provides a method for transforming a plant cell comprising providing a plant cell with a recombinant nucleic acid comprising a T-DNA construct allowing for transfer of said construct into the genome of a plant cell, said construct provided with a foreign nucleic acid that is free of nucleic acid encoding a selective marker.
Claims20
A method for obtaining a market-free, uniform transgenic plant, comprising
- transforming a plant cell with a recombinant nucleic acid comprising a T-DNA construct wherein said T-DNA is provided with a foreign nucleic acid that is free of a nucleic acid encoding a selective marker
- regeneration of said cell under no selective pressure
- testing said cell or progeny thereof for the presence or absence of at least a functional part of said foreign nucleic acid and identify transformed plant cells or progeny thereof
- growing a plant from said identified cell or progeny thereof,
- testing the obtained plant for uniformness and selecting a uniform plant.
A method according to claim 3, wherein said Agrobacterium strain is a virulent Agrobacterium strain.
Dependencies are inferred from the claim text.