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AssigneeBayer BioScience N.V.
InventorsBart Lambert, Stefan Jansens, Katrien Audenhove, Marnix Peferoen
Estimating the term…
Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.
A DNA encoding a variant of the protein of SEQ ID NO:4, having substantially the same insecticidal activity as the protein of SEQ ID NO:4 or an insecticidally-effective fragment thereof, wherein said DNA comprises the nucleotide sequence of SEQ ID NO:2 from nucleotide position 198 to nucleotide position 1561, and the nucleotide sequence of SEQ ID NO:3 from nucleotide position 1 to nucleotide position 1145, or a degenerate DNA sequence thereof, wherein one or more amino acid codons have been replaced with others without changing the amino acid sequence of the protein.
A DNA encoding a protein with insecticidal activity against an insect selected from the group consisting of Spodoptera exigua, Spodoptera littoralis, Spodoptera frugiperda, Agrotis ipsilon, Mamestra brassica, Heliothis virescens, Ostrinia nubilalis, and Plutella xylostella, wherein said DNA comprises the nucleotide sequence of SEQ ID NO:2 from nucleotide position 198 to nucleotide position 1561 or a degenerate DNA sequence thereof wherein one or more amino acid codons have been replaced with others without changing the amino acid sequence of the protein.
A DNA encoding a protein with insecticidal activity against an insect selected from the group consisting of Spodoptera exigua, Spodoptera littoralis, Spodoptera frugiperda, Agrotis ipsilon, Mamestra brassica, Heliothis virescens, Ostrinia nubilalis, and Plutella xylostella, wherein said DNA comprises the nucleotide sequence of SEQ ID NO:3 from nucleotide position 1 to nucleotide position 1145 or a degenerate DNA sequence thereof, wherein one or more amino acid codons have been replaced with others without changing the amino acid sequence of the protein.
Dependencies are inferred from the claim text.