Maize promoter named CRWAQ81

A method for expressing a nucleotide sequence in a plant, said method comprising introducing into a plant cell an expression cassette, said expression cassette comprising a promoter and operably linked to said promoter a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:

• a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2;
• b) a nucleotide sequence comprising a plant promoter sequence of the plasmids designated as Patent Deposit No. PTA-5126;
• c) a nucleotide sequence comprising at least 50 contiguous nucleotides of the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2, wherein said nucleotide sequence initiates transcription in a plant cell;
• d) a nucleotide sequence comprising a sequence having at least 90% sequence identity to the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2, wherein said nucleotide sequence initiates transcription in a plant cell;
• e) a nucleotide sequence comprising a sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2, wherein said nucleotide sequence initiates transcription in a plant cell;
• and,
• f) a nucleotide sequence that hybridizes under stringent conditions to the complement of a) or b), wherein said sequence initiates transcription in a plant cell;
• and,
• regenerating a transformed plant from said plant cell, wherein said plant has stably incorporated into its genome said expression cassette.

A method for expressing a nucleotide sequence in a plant cell, said method comprising introducing into said plant cell an expression cassette comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:

• a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1 or SEQ ID NO:
• 2;
• b) a nucleotide sequence comprising a plant promoter sequence of the plasmids designated as Patent Deposit No. PTA-5126;
• c) a nucleotide sequence comprising at least 50 contiguous nucleotides of the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2, wherein said nucleotide sequence initiates transcription in said plant cell;
• d) a nucleotide sequence comprising a sequence having at least 90% sequence identity to the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2, wherein said nucleotide sequence initiates transcription in said plant cell;
• e) a nucleotide sequence comprising a sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2, wherein said nucleotide sequence initiates transcription in said plant cell;
• and,
• f) a nucleotide sequence that hybridizes under stringent conditions to the complement of a) or b), wherein said sequence initiates transcription in said plant cell.

A method for selectively expressing a nucleotide sequence in a plant root, said method comprising introducing into a plant cell an expression cassette, and regenerating a transformed plant from said plant cell, said expression cassette comprising a promoter and a heterologous nucleotide sequence operably linked to said promoter, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:

• a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2;
• b) a nucleotide sequence comprising a plant promoter sequence of the plasmids deposited as Patent Deposit No. PTA-5126;
• c) a nucleotide sequence comprising at least 50 contiguous nucleotides of the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2, wherein said sequence initiates transcription in a plant root cell;
• d) a nucleotide sequence comprising a sequence having at least 90% sequence identity to the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2, wherein said sequence initiates transcription in a plant root cell;
• e) a nucleotide sequence comprising a sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:1 or SEQ ID NO:2, wherein said sequence initiates transcription in a plant root cell;
• and,
• f) a nucleotide sequence that hybridizes under stringent conditions to the complement of a) or b), wherein said sequence initiates transcription in a plant root cell.