Phenotype modifying genetic sequences
OwnerBernard Carrollinventor
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Abstract
Nucleic acid molecules capable of modifying phenotypic traits in eukaryotic cells and in particular plant cells are described. The nucleic acid molecules of the present invention are referred to as “phenotype modifying genetic sequences” or “PMGSs” and may be used to increase and/or stabilise or otherwise facilitate expression of nucleotide sequences being expressed into a translation product. Alternatively, PMGSs may be used to down regulate by, for example, promoting transcript degradation via mechanisms such as co-suppression. In plant and non-plant cells, the PMGSs of the invention may also be used to inhibit, reduce or otherwise down regulate expression of a nucleotide sequence, such as a eukaryotic gene, including a pathogen gene, the expression of which results in an undesired phenotype.
Claims27
(canceled)
The PMGS according to claim 27 wherein said PMGS promotes demethylation or prevents or inhibits methylation of a second nucleotide sequence located within, adjacent to, or otherwise proximal to said PMGS.
3-6. (canceled)
A genetic construct comprising the PMGS according to claim 27 and means to facilitate insertion of a nucleotide sequence within, adjacent to, or otherwise proximal to said PMGS.
The genetic construct according to claim 29 wherein the inserted nucleotide sequence is operably linked to a promoter.
A method of increasing or stabilizing expression of a nucleotide sequence or preventing or reducing silencing of a nucleotide sequence in a plant, comprising:
- introducing said nucleotide sequence into the plant, such that when expressed in the plant, said nucleotide sequence is flanked by, adjacent to, or otherwise proximal to, the PMGS of claim 27.
10-19. (canceled)
An isolated nucleotide sequence having at least 80% similarity after optimal alignment of said sequence to the PMGS of claim 27, wherein said PMGS Increases or stabilizes the expression of a second nucleotide sequence inserted proximal to said PMGS.
21-22. (canceled)
A sequence capable of hybridizing to the PMGS according to claim 27 under medium stringency conditions at 42° C. comprising from at least about 16% v/v to at least about 30% v/v formamide and from at least about 0.5M to at least about 0.9M salt for hybridization and at least about 0.5M to at least about 0.9M salt for washing.
A sequence capable of hybridizing to the PMGS according to claim 27 under high stringency conditions at 42° C. comprising from at least about 31% v/v to at least about 50% v/v formamide and from at least about 0.01 M to at least about 0.15M salt for hybridization and at least about 0.01M to at least about 0.15M salt for washing.
A method of increasing or stabilizing expression of a nucleotide sequence, or preventing or reducing silencing of a nucleotide sequence, in a plant or plant cell, comprising:
- introducing into said plant or plant cell a construct comprising said nucleotide sequence whose expression is to be increased or stabilized, or whose silencing is to be prevented or reduced, flanked by, adjacent to, or otherwise proximal to the PMGS of SEQ ID NO:12 and SEQ ID NO. 13;
- and
- expressing said construct in said plant or plant cell.
An isolated phenotype modulating genetic sequence (PMGS) comprising the nucleotide sequences of SEQ ID NO:12 and SEQ ID NO:13.
An Isolated nucleic acid comprising the nucleotide sequence of SEQ ID NO:12, SEQ ID NO:13 or both.
A method for inducing or increasing pathogen resistance in a plant which comprises introducing into said plant a genetic construct comprising a PMGS flanked by, adjacent to, or otherwise proximal to a nucleotide sequence that promotes transcript degradation or co-suppression of plant virus, wherein said PMGS comprises SEQ ID NO:12 and SEQ ID NO:13.
Dependencies are inferred from the claim text.